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- 2018
明胶/染料木素纳米复合物的制备及其表征
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Abstract:
摘要 目的:制备结构稳定,易于被包封的明胶/染料木素纳米复合物。方法:两步去溶剂法制备明胶纳米粒(gelatin nanoparticles,GNPs),将染料木素(genistein,GEN)吸附于GNPs表面,形成纳米粒复合物,表征其形态、粒径,明确其吸附条件,并计算包封率;以时间、温度、GNPs的浓度、pH为考量参数,检测上清液在262 nm处的峰值,通过标准曲线方程计算染料木素的浓度,量化各参数对包封率的作用。结果:GNPs微球表面呈锥形或梭形,粒径范围150～200 nm。最高载药量约为34.7%,最优化条件下包封率超过90%。随着纳米复合物的降解,GEN的释放呈现出可逆性,冻干后的纳米复合物,具有良好纳米形态和稳定的药物含量。结论:两步去溶剂法可制备形态均一且具有高包封率的GNPs/GEN纳米复合物,同时具有良好的结构稳定性,可室温保存0.5年以上
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