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- 2018
大鼠微螺钉种植体周围炎外周血中Foxp3+调节性T细胞的水平变化
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Abstract:
摘要 目的:建立大鼠股骨微螺钉种植体周围炎模型,检测其外周血中Foxp3+调节性T细胞水平的变化。方法:12只雄性SD大鼠分为正常组、炎症组、空白组(n=4),正常组和炎症组单侧股骨远心端植入微螺钉种植体1枚,炎症组在植入种植体后用丝线环扎种植体颈部并使种植体上端暴露于体表外;正常组在植入种植体后严密缝合,植体不外露;空白组仅进行麻醉、切开及缝合等对照处理,未植入种植体。术后第4周取样、采血,拍摄X线片并采用流式细胞术检测外周血中Foxp3+调节性T细胞(CD4+CD25+Foxp3+)在T淋巴细胞(CD4+)中的比例。结果: 炎症组大鼠种植体周围出现炎性肉芽组织,X线片显示植体颈部周围皮质骨有角形吸收,其外周血中Foxp3+调节性T细胞占CD4+T淋巴细胞的比例为(0.66±0.32)%,显著低于正常组(2.69±1.19)%和空白组(4.06±1.34)%,差异有统计学意义(P<0.05);正常组和对照组之间差异无统计学意义。结论: 大鼠股骨远心端微螺钉种植体周围炎模型造模成功;该动物模型外周血中Foxp3+调节性T细胞在数量上存在异常,可能与种植体周围炎的发生有关
| [1] | 孙福财,余方友,李贤.盐酸米诺环素软膏对微螺钉支抗种植体周围炎龈下牙龈卟啉单胞菌影响[J].口腔医学研究,2016, 33(4):395-398 |
| [2] | 刘思,吴建勇,刘剑.微螺钉支抗的研究进展[J].南昌大学学报(医学版),2017, 57(2):96-99,106 |
| [3] | Costa N, Pires AE, Gabriel AM, et al. Broadened T-cell repertoire diversity in ivIg-treated SLE patients is also related to the individual status of regulatory T-cells [J]. J Clin Immunol, 2013, 33(2):349-360 |
| [4] | Whiteside TL. Regulatory T cell subsets in human cancer: are they regulating for or against tumor progression [J]. Cancer Immunol Immunother, 2014, 63(1):67-72 |
| [5] | Da Costa A, Garza E, Graham JB, et al. Extrinsic MAVS signaling is critical for Treg maintenance of Foxp3 expression following acute flavivirus infection [J]. Sci Rep, 2017, 7:40720 |
| [6] | 王宇婷,邱憬.免疫应答在种植体周围炎发生发展和防治中作用的研究进展[J].口腔医学,2014, 34(11):857-860 |
| [7] | 郭忠军,周洪,李瑞春,等.微螺钉型种植体支抗对成人双颌前突患者的疗效分析[J].中国口腔颌面外科杂志,2017,15(6):519-523 |
| [8] | 白振西,高志彪,肖霞,等.颌间牵引钉在颌骨骨折内固定手术治疗中的临床应用研究[J].实用口腔医学杂志,2015,31(2):219-224 |
| [9] | 胡超,肖金刚,胡文健,等.颌间牵引钉联合小型或微型钛板内固定修复颌骨骨折[J].中国组织工程研究,2016, 20(11):1610-1615 |
| [10] | 史秋涛,谷志远.肿瘤坏死因子-α与种植体周围炎炎性骨吸收[J].口腔医学研究,2015, 31(7):745-747 |
| [11] | Koivula TT, Tuulasvaara A, Hetem？ki I, et al. Regulatory T cell response correlates with the severity of human hantavirus infection [J]. J Infect, 2014, 68(4):387-394 |
| [12] | Wang X, Lang M, Zhao T, et al. Cancer-FOXP3 directly activated CCL5 to recruit FOXP3(+)Treg cells in pancreatic ductal adenocarcinoma [J]. Oncogene, 2017, 36(21):3048-3058 |
| [13] | Lu L, Barbi J, Pan F. The regulation of immune tolerance by FOXP3 [J]. Nat Rev Immunol, 2017, 17(11):703-717 |
| [14] | 王琳源,关宁,林晓萍.Treg 细胞介导的免疫应答在牙周炎小鼠中的研究[J].实用口腔医学杂志,2015, 31(3):318-322 |
| [15] | 李喜红,王淑敏,崔淑霞.国产自攻型微螺钉种植体感染的实验研究[J].中国口腔种植学杂志,2013,18(3):122-124 |
| [16] | 张燎,于宝军.PRP对微螺钉种植支抗加载后不同时期周围骨组织变化的实验研究[J].中国口腔种植学杂志,2015,20(3):106-110 |
| [17] | Okui T, Aoki Y, Ito H, et al. The presence of IL-17<sup>+</sup>/FOXP3<sup>+</sup> double-positive cells in periodontitis [J]. J Dent Res, 2012, 91(6):574-579 |
| [18] | 孙志新,张云涛.口腔种植体周围炎与白细胞介素间的关系[J].国际口腔医学杂志,2015, 42(2):221-224 |
| [19] | Sun H, Gao W, Pan W, et al. Tim3(+) Foxp3 (+) Treg cells are potent inhibitors of effector T cells and are suppressed in rheumatoid arthritis [J]. Inflammation, 2017, 40(4):1342-1350 |
| [20] | Naufel AO, Aguiar MCF, Madeira FM, et al. Treg and Th17 cells in inflammatory periapical disease: a systematic review [J]. Braz Oral Res, 2017, 31:e103 |
| [21] | 滕丹丽, 梁文红.Treg细胞在口腔疾病中的研究进展[J].中国医学创新,2015,12(12):136-139 |
| [22] | Hussaini HM, Parachuru VPB, Seymour GJ, et al. FoxP3 and TLR2 in co-expression in oral cancer [J]. Acta Histochem, 2017, 119(7):768 |
| [23] | 赵川江,徐琛蓉,周玉竹,等.侵袭性牙周炎患者外周血CD-4<sup>+</sup>CD-25<sup>+</sup>调节性T细胞的检测及功能分析 [J]. 中华口腔医学杂志, 44(7):413-415 |
| [24] | 徐琛蓉,赵川江,江丽.慢性牙周炎病人外周血CD4<sup>+</sup>CD25<sup>+</sup>调节性T细胞的检测和免疫抑制功能分析[J].牙体牙髓牙周病学杂志,2009, 19(9):493-496 |
| [25] | Boduc M, Roessler M, Mandic R, et al. Foxp3 expression in lymph node metastases in patients with head and neck cancer [J]. Acta Otolaryngol, 2017, 137(11):1215-1219 |
| [26] | 何昇,杨璐铭,李运峰,等.骨质疏松对完成骨整合的大鼠股骨钛种植体稳定性的影响[J].口腔医学,2017,37(11):991-996 |
