|
|
- 2018
透明质酸对IL-1β刺激下髁突软骨细胞中HMGB-1表达的影响
|
Abstract:
摘要 目的: 研究透明质酸对IL-1β刺激下髁突软骨细胞中HMGB-1表达的影响。方法: 体外分离培养大鼠髁突软骨细胞,将透明质酸(hyaluronic acid,HA)和甘草酸(glycyrrhizin,GL)分别与IL-1β同时加入髁突软骨细胞中,刺激24 h后提取各组细胞总RNA,检测HMGB-1及其相关受体基因表达;提取细胞总蛋白检测HMGB-1蛋白表达。结果: IL-1β刺激髁突软骨细胞24 h后 HMGB-1、TLR2和TLR4基因表达显著升高(P<0.05);IL-1β与HA或GL同时加入刺激的软骨细胞与仅加入IL-1β刺激组相比,HMGB-1、TLR2和TLR4的基因表达较的表达显著降低,HMGB-1蛋白表达也显著降低(P<0.05)。结论: HA可降低IL-1β刺激后髁突软骨细胞中HMGB-1及其受体TLR2、TLR4的表达
| [1] | Maharjan AS, Pilling D, and Gomer RH. High and low molecular weight hyaluronic acid differentially regulate human fibrocyte differentiation [J]. PLoS One, 2011, 6(10)∶e26078 |
| [2] | Liu-Bryan R. and Terkeltaub R. Chondrocyte innate immune myeloid differentiation factor 88-dependent signaling drives procatabolic effects of the endogenous Toll-like receptor 2/Toll-like receptor 4 ligands low molecular weight hyaluronan and high mobility group box chromosomal protein 1 in mice [J]. Arthritis Rheum, 2010, 62(7)∶2004-2012 |
| [3] | Park SY, Lee SW, Kim HY, et al. HMGB1 induces angiogenesis in rheumatoid arthritis via HIF-1α activation [J]. European Journal of Immunology, 2015, 45(4)∶1216-1227 |
| [4] | Li C, Long X, Deng M, et al. Osteoarthritic changes after superior and inferior joint space injection of hyaluronic acid for the treatment of temporomandibular joint osteoarthritis with anterior disc displacement without reduction: a cone-beam computed tomographic evaluation [J]. J Oral Maxillofac Surg, 2015, 73(2)∶232-244 |
| [5] | Wahamaa H, Schierbeck H, Hreggvidsdottir HS, et al. High mobility group box protein 1 in complex with lipopolysaccharide or IL-1 promotes an increased inflammatory phenotype in synovial fibroblasts [J]. Arthritis Res Ther, 2011, 13(4)∶R136 |
| [6] | Varoga D, Paulsen F, Mentlein R, et al. TLR-2-mediated induction of vascular endothelial growth factor (VEGF) in cartilage in septic joint disease [J]. J Pathol, 2006, 210(3)∶315-324 |
| [7] | Dicker KT, Gurski LA, Pradhan-Bhatt S, et al. Hyaluronan: A simple polysaccharide with diverse biological functions [J]. Acta Biomaterialia, 2014, 10(4)∶1558-1570 |
| [8] | Ferrari S, Ronfani L, Calogero S, et al. The mouse gene coding for high mobility group 1 protein (HMG1) [J]. J Biol Chem, 1994, 269(46)∶28803-28808 |
| [9] | Taniguchi N, Kawahara K, Yone K, et al. High mobility group box chromosomal protein 1 plays a role in the pathogenesis of rheumatoid arthritis as a novel cytokine [J]. Arthritis Rheum, 2003, 48(4)∶971-981 |
| [10] | Feng Y, Fang W, Li C, et al. The expression of high-mobility group box protein-1 in temporomandibular joint osteoarthritis with disc perforation [J]. J Oral Pathol Med, 2016, 45(2)∶148-152 |
| [11] | Li C, Cai H, Meng Q, et al. IL-1beta mediating high mobility group box protein-1 expression in condylar chondrocyte during temporomandibular joint inflammation [J]. J Oral Pathol Med, 2016, 45(7)∶539-545 |
| [12] | 袁健,龚忠诚,凌彬,等.透明质酸钠关节下腔注射治疗颞下颌关节盘前移位的疗效分析[J].口腔医学研究,2012,28(11)∶1145-1148 |
| [13] | Heinola T, de Grauw JC, Virkki L, et al. Bovine chronic osteoarthritis causes minimal change in synovial fluid [J]. J Comp Pathol, 2013, 148(4)∶335-344 |
| [14] | Campo GM, Avenoso A, D'Ascola A, et al. Hyaluronan differently modulates TLR-4 and the inflammatory response in mouse chondrocytes [J]. Biofactors, 2012, 38(1)∶69-76 |
| [15] | Julovi SM, Ito H, Nishitani K, et al. Hyaluronan inhibits matrix metalloproteinase-13 in human arthritic chondrocytes via CD44 and P38 [J]. J Orthop Res, 2011, 29(2)∶258-264 |
| [16] | Mladenovic Z, Saurel AS, Berenbaum F, et al. Potential role of hyaluronic acid on bone in osteoarthritis: matrix metalloproteinases, aggrecanases, and RANKL expression are partially prevented by hyaluronic acid in interleukin 1-stimulated osteoblasts [J]. J Rheumatol, 2014. 41(5)∶945-954 |
