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- 2018
黄瓜籽正丁醇提取物对人牙髓干细胞增殖及成牙向分化的影响
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Abstract:
摘要 目的:探讨黄瓜籽正丁醇提取物(n-butanol extract of cucumis sativus seeds,CSB)对人牙髓干细胞(human dental pulp stem cells,hDPSCs)增殖及成牙向分化能力的影响。方法:体外扩增培养hDPSCs,采用细胞计数法(cell counting kit-8,CCK-8)法检测CSB对hDPSCs增殖能力的影响;通过碱性磷酸酶(ALP)染色及活性测定、Western blot检测牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)和Ⅰ型胶原(collagen Ⅰ,COL Ⅰ)等矿化相关蛋白的表达水平观察细胞成牙向分化能力的变化。结果:CCK-8结果显示,与对照组相比,100 mg/L CSB可显著促进hDPSCs的增殖(P<0.05),500 mg/L组则明显抑制hDPSCs的增殖(P<0.05);经矿化诱导后,100 mg/L CSB组ALP染色明显加深,活性显著升高(P<0.05);Western blot结果显示100 mg/L CSB组DSPP和COL Ⅰ的表达均高于对照组(P<0.05)。结论:适宜浓度的CSB可促进hDPSCs的增殖及成牙向分化
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