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- 2017
电离辐射对大鼠颌下腺钙离子激活型钾通道的影响
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Abstract:
摘要 目的:通过研究电离辐射对大鼠颌下腺腺泡细胞膜上BK通道和IK通道开放的影响,探讨照射后唾液腺分泌功能降低的可能分子机制。方法:将大鼠随机分为对照组和照射组,对照射组大鼠的颌下腺区一次性15 Gy X线照射,对照组不照射。分别在照射后3 d、15 d和40 d取出大鼠颌下腺,胶原蛋白酶消化,从每只大鼠颌下腺消化液中挑选1个状态好的细胞,全细胞膜片钳技术研究大鼠颌下腺腺泡细胞膜上BK和IK1通道电流的变化。结果:1)在+80 mV时,相比对照组大鼠颌下腺腺泡细胞膜上BK通道电流密度,照射后3 d没有显著性差异,照射后15 d减少9.7%(P<0.05),照射后40 d减少13.8%(P<0.01)。2)在+80 mV时,相比对照组大鼠颌下腺腺泡细胞膜上IK1通道电流密度,照射后3d没有显著性差异,照射后15 d减少12.8%(P<0.05),照射后40 d减少17.1%(P<0.01)。结论:电离辐射所致的大鼠唾液腺分泌功能减退可能与辐射后大鼠颌下腺腺泡细胞膜上BK和IK1通道开放电流减少导致的K+外流减少有关
| [1] | Feng B, Ye W L, Ma L J, et al. Hydrogen peroxide enhanced Ca<sup>2+</sup>-activated BK currents and promoted cell injury in human dermal fibroblasts [J]. Life Sciences, 2012, 90(11-12)∶424-431 |
| [2] | Urek MM, Bralic M, Tomac J, et al. Early and late effects of X-irradiation on submandibular gland: a morphological study in mice [J]. Arch Med Res, 2005, 36(4)∶339-343 |
| [3] | Romanenko VG, Nakamoto T, Srivastava A, et al. Regulation of membrane potential and fluid secretion by Ca<sup>2+</sup>-activated K<sup>+</sup> channels in mouse submandibular glands [J]. J Physiol, 2007, 581(Pt 2)∶801-817 |
| [4] | Zhijian Wang, Arohan R. Subramanya, et al. Regulation of large-conductance Ca<sup>2+</sup>-activated K<sup>+</sup> channels by WNK4 kinase [J]. American journal of physiology. Cell physiology, 2013, 305(8)∶846-853 |
| [5] | Vissink A, Mitchell JB, Baum BJ, et al. Clinical management of salivary gland hypofunction and xerostomia in head-and-neck cancer patients: successes and barriers [J]. Int J Radiat Oncol Biol Phys, 2010, 78(4)∶983-991 |
| [6] | Yang H, Zhang G, Cui J. BK channels: multiple sensors, one activation gate [J]. Frontiers in Physiology, 2015, 6(1)∶29-35 |
| [7] | Zhou XB, Wulfsen I, Utku E, et al. Dual role of protein kinase C on BK channel regulation [J]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(17)∶8005-8010 |
| [8] | Vetri F, Xu H, Paisansathan C, et al. Impairment of neurovascular coupling in type 1 diabetes mellitus in rats is linked to PKC modulation of BK(Ca) and Kir channels [J]. Ajp Heart & Circulatory Physiology, 2012, 302(6)∶H1274-84 |
| [9] | Almassy J, Won JH, Begenisich TB, et al. Apical Ca<sup>2+</sup>-activated potassium channels in mouse parotid acinar cells [J]. J Gen Physiol, 2012, 139(2)∶121-133 |
| [10] | Soloviev A, Tishkin S, Kyrychenko S. Quercetin-filled phosphatidylcholine liposomes restore abnormalities in rat thoracic aorta BK(Ca) channel function following ionizing irradiation [J]. Sheng Li Xue Bao, 2009, 61(3)∶201-210 |
| [11] | Kunduri SS, Dick GM, Nayeem MA, et al. Adenosine A1 receptor signaling inhibits BK channels through a PKCα-dependent mechanism in mouse aortic smooth muscle [J]. Physiological Reports, 2013, 1(3). pii∶e00037 |
