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- 2018
芦荟大黄素纳米脂质体介导的光动力疗法对人舌鳞状细胞癌细胞Tca-8113治疗效果的研究
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Abstract:
摘要 目的: 探究芦荟大黄素纳米脂质体(aloe emodin nano-liposome,AE-L)介导的光动力疗法(photodynamic therapy,PDT)对人舌鳞状细胞癌(简称鳞癌)细胞Tca-8113的治疗效果。方法: 逆相蒸发法制备AE-L;不同浓度AE-L(2.5、5.0、7.5、10.0、12.5 μg/mL)作用Tca-8113细胞4 h后,不同光照时间处理(0、10、20、40、80、160 s;激发波长为405 nm,功率密度为80 mW/cm2)Tca-8113,采用噻唑蓝(methyl thiazol tetrazolium,MTT)检测细胞存活率;AE-L(7.5 μg/mL)作用细胞4 h后,光照时间为80 s(激发波长为405 nm,功率密度为80 mW/cm2)处理细胞,荧光显微镜定性分析PDT后活性氧(reactive oxygen species,ROS)含量。结果: AE-L浓度≤7.5 μg/mL时细胞毒性较小,与对照组比较差异无统计学意义(P＞0.05)。PDT组随着光照时间延长细胞存活率逐渐降低,光照时间80 s时细胞存活率为76.6%,与对照组比较差异有统计学意义(P＜0.001)。荧光染色结果显示,PDT治疗后细胞内ROS含量显著增加。结论: 7.5 μg/mL AE-L介导的PDT治疗对人舌鳞癌Tca-8113细胞具有显著的杀伤效果
| [1] | Khangura SK, Greenwald BD. Endoscopic management of esophageal cancer after definitive chemoradiotherapy [J] . Dig Dis Sci,2013,58(6)∶1477-1485 |
| [2] | Jerjes W, Hamdoon Z, Hopper C. Photodynamic therapy in the management of potentially malignant and malignant oral disorders [J] . Head Neck Oncol,2012,4∶17 |
| [3] | Usuda J,Ishizumi T,Inoue T,et al. Photodynamic diagnosis (PDD) for central type lung cancers [J] . J Nippon Med Sch,2013,80(6)∶402-403 |
| [4] | 赵凌冰,王新星,李晓梅,等.P-gp和ABCG2在口腔鳞状细胞癌中的表达及临床病理意义[J] .口腔医学研究,2012,28(1)∶32-35 |
| [5] | Sun Y, Xing D, Shen L, et al. Bactericidal effects of hematoporphyrin monomethyl ether-mediated photosensitization against pathogenic communities from supragingival plaque [J] . Appl Microbiol Biotechnol, 2013, 97(11)∶5079-5087 |
| [6] | Matsukawa S,Morita K,Negishi A, et al.Galectin-7 as a potential predictive marker of chemo-and/or radio-therapy resistance in oral squamous cell carcinoma [J] . Cancer Med, 2014, 3(2)∶349-361 |
| [7] | 夏冬景,黄莹莹,李海朋,等.磷酸二酯酶4D在口腔鳞状细胞癌发生发展过程中的作用研究[J] .口腔医学研究,2017,33(6)∶663-667 |
| [8] | Crombie AK,Farah C,Tripcony L,et al. Primary chemoradiotherapy for oral cavity squamous cell carcinoma [J] . Oral Oncol,2012,48(10)∶1014-1018 |
| [9] | Manor E, Tetro S. Translocation (12;14) and other chromosome abnormalities in squamous cell carcinoma of the tongue [J] . Eur Arch Otorhinolaryngol, 2010, 267(8)∶1273-1276 |
| [10] | Kwitniewski M, Juzeniene A, Ma LW, et al. Diamino acid derivatives of PpIX as potential photosensitizers for photodyamic therapy of squamous cell carcinoma and prostate cancer:<em>in vitro</em> studies [J] . J Photochem Photobiol B,2009,94(3)∶214-222 |
| [11] | 段勤勤,白定群,李开庭,等.AE-L介导的光动力对胃癌细胞的作用[J] .重庆医科大学报,2014,39(3)∶305-309 |
| [12] | Planka J, Latocha M. Photodynamic therapy in the treatment of breast cancer [J] . Pol Merkur Lekarski,2012,33(195)∶173-175 |
| [13] | Patrick Ross Jr. Photodynamic therapy for airway malignancies: the Ohio State University experience since [J] . J Natl Compr Canc Netw, 2012, 10 Suppl 2∶S9-S13 |
| [14] | Calzavara PP, Rossi MT, Sala R. Photodynamic antifungal chemotherapy [J] . Photochem Photobiol,2012,88(3)∶512-522 |
| [15] | Hinger D, Gr fe S, Navarro F, et al. Lipid nanoemulsions and liposomes improve photodynamic treatment efficacy and tolerance in CAL-33 tumor bearing nude mice [J] . J Nanobiotechnology, 2016, 14(1)∶71 |
