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- 2018
白花丹素对舌鳞状细胞癌细胞的侵袭和迁移的作用及机制研究
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Abstract:
摘要 目的:探讨葡萄糖转运蛋白1(glucose transporter isoform 1,GLUT1)与舌鳞状细胞癌(tongue squamous cell carcinoma,TSCC)转移的关系,以及白花丹素(plumbagin,PLB)对TSCC细胞侵袭和迁移的影响及作用机制。方法:免疫组化分析GLUT1和基质金属蛋白酶 2(matrix metalloprotein 2,MMP2)与TSCC转移的关系。MTT检测PLB对TSCC细胞的增殖抑制作用,并计算24 h的IC50值。Transwell、划痕实验和Westernblot分别检测肿瘤细胞的侵袭和迁移能力以及相关蛋白的表达改变。结果:免疫组化显示GLUT1和MMP2的高表达与TSCC的转移密切相关(P<0.05)。MTT显示PLB作用24 h的IC50值为6.87 μmol/L;Transwell以及划痕实验分别显示,PLB及NSC207895、WZB117处理组的细胞穿膜数及迁移率明显减少(P＜0.05);Westernblot显示,PLB处理后p53表达升高(P＜0.05)、GLUT1以及MMP2表达下降(P＜0.05);NSC207895处理后GLUT1及MMP2表达下降(P＜0.05);WZB117处理后p53无明显改变(P＞0.05)、MMP2表达下降(P＜0.05)。结论:GLUT1高表达与TSCC转移密切相关,PLB可通过调控p53/GLUT1/MMP2通路抑制TSCC细胞的侵袭和迁移
| [1] | Schwartzenberg-Bar-Yoseph F, Armoni M, Karnieli E. The tumor suppressor p53 down-regulates glucose transporters GLUT1 and GLUT4 gene expression [J]. Cancer Res,2004,64(7)∶2627-2633 |
| [2] | van der Waal I. Are we able to reduce the mortality and morbidity of oral cancer; some considerations [J]. Med Oral Patol Oral Cir Bucal,2013,18(1)∶e33-e37 |
| [3] | Hanahan D, Weinberg R A. Hallmarks of cancer: the next generation [J]. Cell,2011,144(5)∶646-674 |
| [4] | Yu M, Yongzhi H, Chen S, et al. The prognostic value of GLUT1 in cancers: a systematic review and meta-analysis [J]. Oncotarget,2017,8(26)∶43356-43367 |
| [5] | 黄喆,高志彪,冯正虎,等.MMP-9在不同浸润方式舌鳞状细胞癌中的表达及临床意义[J].口腔医学研究,2012,28(5)∶488-490 |
| [6] | Oh S, Kim H, Nam K, et al. Glut1 promotes cell proliferation, migration and invasion by regulating epidermal growth factor receptor and integrin signaling in triple-negative breast cancer cells [J]. BMB Rep,2017,50(3)∶132-137 |
| [7] | Xu Y Y, Bao Y Y, Zhou S H, et al. Effect on the expression of MMP-2, MT-MMP in laryngeal carcinoma Hep-2 cell line by antisense glucose transporter-1 [J]. Arch Med Res,2012,43(5)∶395-401 |
| [8] | Koch A, Lang S A, Wild P J, et al. Glucose transporter isoform 1 expression enhances metastasis of malignant melanoma cells [J]. Oncotarget,2015,6(32)∶32748-32760 |
| [9] | Remmele W, Stegner H E. Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue [J]. Der Pathologe,1987,8(3)∶138 |
| [10] | Vander H M, Cantley L C, Thompson C B. Understanding the Warburg effect: the metabolic requirements of cell proliferation [J]. Science,2009,324(5930)∶1029-1033 |
| [11] | Kitamura K, Hatano E, Higashi T, et al. Proliferative activity in hepatocellular carcinoma is closely correlated with glucose metabolism but not angiogenesis [J]. J Hepatol,2011,55(4)∶846-857 |
| [12] | Mueckler M, Thorens B. The SLC2 (GLUT) family of membrane transporters [J]. Mol Aspects Med,2013,34(2-3)∶121-138 |
| [13] | Ruan M, Zhang Z, Li S, et al. Activation of Toll-like receptor-9 promotes cellular migration via up-regulating MMP-2 expression in oral squamous cell carcinoma [J]. PLoS One,2014,9(3)∶e92748 |
