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- 2017
介孔硅载七叶皂苷钠对粪肠球菌抑菌作用的研究
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Abstract:
摘要 目的: 探讨氨基改性前后的介孔硅载七叶皂苷钠(SA-SBA-15及SA-NH2-SBA-15)和氢氧化钙Ca(OH)2对粪肠球菌的抑菌作用。方法: 液体稀释法测SA-NH2-SBA-15、SA-SBA-15和Ca(OH)2的最低抑菌浓度(MIC);液体扩散法观察SA-NH2-SBA-15、SA-SBA-15和Ca(OH)2对粪肠球菌抑菌圈的大小;SA-NH2-SBA-15、SA-SBA-15和Ca(OH)2与菌液共培养168 h,每隔24 h取0.1mL液体另作厌氧培养24 h,评价抑菌效果。结果: SA-NH2-SBA-15、SA-SBA-15的MIC为3.5 g/L,Ca(OH)2的MIC为4.0 g/L。SA-NH2-SBA-15、SA-SBA-15和Ca(OH)2浓度大于MIC时,抑菌圈大小与浓度呈依赖性增高,差异具有统计学意义(P＜0.05)。72 h后至168 h,SA-SBA-15和SA-NH2-SBA的菌落数明显少于Ca(OH)2。96 h后,SA-NH2-SBA-15组的菌落数明显少于SA-SBA-15组,差异具有统计学意义(P＜0.05)。结论: SA-NH2-SBA-15、SA-SBA-15及Ca(OH)2对粪肠球菌具有一定的抗菌作用;SA-NH2-SBA-15、SA-SBA-15的抑菌效果优于Ca(OH)2;SA-NH2-SBA-15对粪肠球菌的药效长于SA-SBA-15,长于Ca(OH)2
| [1] | 范鹏,范永震,许颖,等.水溶性蜂胶对感染根管内粪肠球菌的体外抑菌试验[J].中国微生态学杂志,2011,23(2)∶144-146 |
| [2] | 熊艳,李海池,彭银等.娑罗子中七叶皂苷类成分的最新研究进展[J].中药材,2016,39(5)∶1195-1199 |
| [3] | 宋晓虹,王维皓,陈世涛.高效液相色谱-离子阱质谱分析七叶皂苷钠中的皂苷类成分[J].中国中药杂志,2016,41(13)∶2449-2454 |
| [4] | Li W,Liu H,Xu Q. Extracellular dextran and DNA affect the formation of Enterococcus faecalis biofilms and their susceptibility to 2% chlorhexidine [J]. J Endod,2012,38(7)∶894-898 |
| [5] | Heyder M, Kranz S, V lpel A, et al. Antibacterial effect of different root canal sealers on three bacterial species [J]. Dent Mater, 2013,29(5)∶542-549 |
| [6] | Kim D,Kim E. Antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment: a literature review-PartⅠ.In vitro studies [J]. Restor Dent Endod,2014,39(4)∶241-252 |
| [7] | X Wang,KSK Lin,JCC Chan, et al. Direct synthesis and catalytic applications of ordered large pore aminopropyl-functionalized SBA-15 mesoporous materials [J]. J. Phys.Chem, 2005, 109(1)∶1763-1769 |
| [8] | 刘琨,侯本祥.粪肠球菌和变异链球菌脂磷壁酸的生物学活性[J].国际口腔医学杂志,2017,44(1)∶118-123 |
| [9] | 孙慧斌,邓倩,王云,等.根管治疗失败病例根管内微生物的分子生物学检测[J].实用口腔医学杂志,2010,26(1)∶96-99 |
| [10] | hu X, Wang Q, Zhang C, et al. Prevalence, phenotype, and genotype of nterococcus faecalis from saliva and root canals in patients with persistent apical periodontitis [J]. J Endod, 2010, 36(12)∶1950-1955 |
| [11] | 梁伟腾.站污层对大蒜素和氢氧化钙抗菌活性影响的体外研究[D].中南大学,2011 |
| [12] | 张琛,杨玥,侯本祥.持续性根尖周炎优势菌粪肠球菌生物膜形成能力的体外研究[J].北京口腔医学,2016(1)∶1-5 |
| [13] | Turk BT, Sen BH, Ozturk T. In vitro antimicrobial activity of calcium hydroxide mixed with different vehicles against Enterococcus faecalis and Candida albicans [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2009, 108(2)∶297-301 |
| [14] | V.Ambrogia, L.Perioli. Improvement of dissolution rate of piroxicam by inclusion into MCM-41 mesoporous silicate [J]. Eur.J.Pharm.Sci, 2007, 32(2)∶216-222 |
| [15] | M.V.Speybroeck, V.Barillaro, et al. Ordered mesoporous silica materials SBA-15: A broad spectrum formulation platform for poorly soluble drugs [J]. Eur.J.Pharm.Sci, 2008, 98(1)∶2648-2658 |
| [16] | 王祥柱,谢晓莉,陈敏慜,等.大蒜素标准品DSTS体外抗粪肠球菌生物膜作用的研究[J].口腔医学研究,2015,31(9)∶867-870 |
| [17] | M.Vallet-Regi, L.Ruiz-Gonzalez,I Izquierdo-Barba, et al. Revisiting silica based ordered mesoporous materials: medical applications [J]. J.Mater.Chem, 2006,16(1)∶26-31 |
| [18] | 任毅,文华,李远.七叶皂苷钠治疗混合痔术后创面水肿的临床疗效观察[J].世界中医药, 2012,7(5)∶398-400 |
| [19] | K.Unger, H.Rupprecht, B. Valentin, et al. The use of porous and surface modified silicas as drug delivery and stabilizing agents [J]. Drug Dev. Ind. Pharm, 1983, 9(1)∶69-91 |
| [20] | Ferrari PH,Gai S,Bombana AC,et al. Effect of endodontic procedures on enterococci,enteric bacteria and yeasts in primary endodontic infections [J]. Int Endod J, 2005, 38(6)∶372-380 |
| [21] | Q. Yang, S. Wang, P. Fan, et al. pH-responsive carrier system based on carboxylic acid modified mesoporous silica and polyelectrolyte for drug delivery [J]. Chem. Mater, 2005, 17(1)∶5999-6003 |
| [22] | 刘振宇.氢氧化钙碘甘油糊剂作为根管内封药的疗效观测[J].中国保健营养,2012,6 (4)∶45-46 |
| [23] | 张富华,李矛,韦智君.纳米银颗粒联合氢氧化钙对饥饿期粪肠球菌生物膜的作用[J].上海口腔医学,2016,(1)∶11-15 |
| [24] | KY Ho,A Gordon Mckay,KL Yeung. Selective adsorbents from ordered mesoporous silica [J]. Langmuir, 2003, 19(1)∶3019-3024 |
| [25] | 李乐园.氨基功能化SBA-15对Cr~(3+),Zn~(2+)吸附性能研究[D].齐齐哈尔大学,2012 |
| [26] | 倪敏. 布洛芬/介孔硅纳米缓控释药物的超临界制备及其释放特性研究[D].大连理工大学,2012 |
| [27] | 阿麦吉.大蒜素对根尖周炎渗出液中IL-1β和TNF-α表达的影响[D].中南大学,2013 |
| [28] | WANG Y, HAN N, ZHAO Q F, et al. Redox-responsive mesoporous silica as carriers for controlled drug delivery: a comparative study based on silica and PEG gatekeepers [J]. European Journal of Pharmaceutical Sciences, 2015, 72(2)∶12-20 |
