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- 2018
原位法合成纳米银对树脂粘结剂抗菌改性的应用研究
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Abstract:
摘要 目的:运用原位法合成纳米银对树脂粘结剂进行抗菌改性,并评价其抗菌性能。方法:将2-乙基己酸银添加到甲基丙烯酸叔丁基氨基乙酯中形成8wt%的银源溶液,再将其添加到Clearfil SE Bond的树脂粘结剂中,获得含2-乙基己酸银浓度为0wt%、0.10wt%、0.20wt%的树脂粘结剂。将树脂粘结剂制成直径为6 mm,厚度为1 mm的试件,再分别接受20 s,1 000 mW/cm2光强度照射完成固化。用透射电镜观察固化后试件中纳米银的生成及分散情况,粒径大小及分布;将试件分别浸泡在无菌人工唾液中1 d和1周,采用直接接触法检测固化后试件表面对变形链球菌的抗菌活性,并采用荧光共聚焦显微镜观察其对细胞胞膜的影响。结果:透射电镜观察到0.10wt%和0.20wt%组试件中有纳米颗粒生成,分散均匀,平均粒径分别为(3.12±0.82) nm,(3.64±0.65) nm;0wt%、0.10wt%和0.20wt%组试件浸泡1 d后均有显著的抗菌活性,且抗菌率无显著差异;浸泡1周后,0wt% 组抗菌率降低,0.10wt%和0.20wt%组抗菌率提高,并显著高于0wt%组的。结论:原位法合成纳米银在树脂粘结剂的抗菌改性中的应用是可行的
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