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- 2018
地塞米松诱导小鼠腭裂的母体血浆代谢组学分析
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Abstract:
摘要 目的:应用代谢组学技术比较地塞米松干预后孕鼠与对照孕鼠在胚胎腭突发育期间的血浆代谢产物的差异,为发现地塞米松致畸时特征性代谢产物奠定一定基础。方法:将孕鼠随机分为地塞米松组(实验组)和生理盐水对照组,特定孕期分别给实验组和对照组小鼠腹腔注射一定量的地塞米松和生理盐水,并于胚胎17.5 d收集孕鼠血浆样本并统计胎鼠腭裂发生率。应用核磁共振波谱仪获取孕鼠血浆样本的核磁共振氢谱图,结合主成分分析法和偏最小二乘法描绘实验组和对照组孕鼠血浆代谢产物在胚胎腭突发育阶段的代谢差异,获得地塞米松致腭裂的特异性代谢标志物。结果:实验组和对照组孕鼠在胚胎腭突发育期间血浆代谢产物有差异。结论:地塞米松可能通过引起胚胎腭突融合阶段氨基酸信号的改变,启动相关调控机制使胚胎发育延缓而导致腭裂的发生
| [1] | A Kidiyoor , SV Murphy , A Atala.Cell and molecular biology and imaging of stem cells: stem cells from the amniotic fluid and placenta [J]. Cell and Molecular Biology and Imaging of Stem Cells,2014∶1-20 |
| [2] | 周京琳.基于代谢组学的地塞米松诱导子鼠腭裂与母鼠孕期代谢物变化相关性研究[D].四川大学华西口腔医学院,2009 |
| [3] | Lan SJ, Yang XG, Chen Z, et al. Role of GATA-6 and bone morphogenetic protein-2 in dexamethasone-induced cleft palate formation in institute of cancer research mice [J]. J Craniofac Surg,2016,27(6)∶1600-1605 |
| [4] | Q Zheng, MA L, LI L.Down-regulation of PAR/aPKC complex in dexamethasone-induced cleft palate [J]. International Journal of Oral & Maxillofacial Surgery,2015(44)∶e315-e316 |
| [5] | 庞骁霄,李丽,马利,等.地塞米松诱导小鼠腭裂及E-钙黏素基因表达的研究[J].华西口腔医学杂志,2015,33(6)∶581-584 |
| [6] | Sheedy JR. Metabolite analysis of biological fluids and tissues by proton nuclear magnetic resonance spectroscopy [J]. Methods Mol Biol,2013,1055:81-97 |
| [7] | 蔡尚宏.唇腭裂相关病因学研究进展[J].现代医药卫生,2016,32(12)∶1839-1841 |
| [8] | 徐滨,周京琳,何苇,等.地塞米松致C57BL/6J小鼠胚胎腭裂的代谢物组1H-NMR模式识别探讨[J].华西口腔医学杂志,2009,26(2)∶367-370 |
| [9] | Guo JF, Xie RH. Numerical simulation and parameter analysis of NMR T2-D distributions of tight sandstone saturated with a gas-water two-phase fluid [J]. Journal of Natural Gas Science and Engineering,2017,(37)∶502-511 |
| [10] | Serrano MJ, Liu J, Svoboda KK. Ephrin reverse signaling mediates palatal fusion and epithelial-to-mesenchymal transition independently of tgfβ3 [J]. J Cell Physiol,2015,230(12)∶2961-2972 |
| [11] | Bernardo JD, Kunisak SM.Amniotic fluid stem cell populations [J]. Fetal Stem Cells in Regenerative Medicine,2016:167-179 |
| [12] | 王辉,库莉博,徐国权,等.1HNMR代谢组学方法对牙周炎患者唾液代谢轮廓的分析 [J].口腔医学研究,2017,33(4)∶412-415 |
| [13] | Xu J, Liu H, Lan Y, et al. Shh-Foxf-Fgf18-Shh molecular circuit regulating palate development [J]. PLoS Genet,2016,12(1)∶e1005769 |
| [14] | Arisa Higa, Kyoko Oka, Michiko Kira-Tatsuoka, et al. Intracellular Signaling Pathway Activation via TGF-β Differs in the Anterior and Posterior Axis During Palatal Development [J]. Journal of Hard Tissue Biology,2016,25(2)∶195-204 |
| [15] | 胡晓.环境因素(地塞米松、维甲酸和二噁英)诱导腭裂的发病机制[D].南方医科大学,2013 |
| [16] | 王睿柴.栋临床药理学新编[M].北京:人民军医出版社,2007 |
| [17] | Sasaki Y, Taya Y, Saito K, et al. Molecular contribution to cleft palateproduction in cleft lip mice [J]. Congenit Anom(Kyoto),2014,54(2)∶94-99 |
