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- 2018
沉默信息调节因子1在颞下颌关节骨关节炎滑膜中表达的相关研究
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Abstract:
摘要 目的:探讨沉默信息调节因子1(silent information regulator 1,SIRT1)在颞下颌关节骨关节炎(temporomandibular joint osteoarthritis,TMJ OA)滑膜组织细胞中表达的差异性。方法:苏木素-伊红染色观察滑膜组织形态,免疫组织化学染色法检测滑膜组织SIRT1表达及分布情况;滑膜细胞体外培养传代,Western blot及实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,Real-time PCR)法检测滑膜细胞中SIRT1蛋白和基因表达。结果:苏木素-伊红染色结果显示TMJ OA组滑膜较正常组明显增厚,血管增多;免疫组织化学染色法结果显示SIRT1在TMJ OA组染色强度较正常组明显升高(P<0.05);Real-time PCR及Western blot结果显示TMJ OA组滑膜细胞中SIRT1表达明显高于正常组(P<0.05)。结论: SIRT1在TMJ OA滑膜中表达升高,与骨关节炎的发生及发展密切相关,为该病发生的分子机制以及治疗提供一个新的理论依据
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