|
|
- 2017
口腔鳞癌新辅助化疗前后多药耐药相关蛋白的表达及临床疗效分析
|
Abstract:
摘要 目的: 观察口腔鳞癌患者新辅助化疗疗效与5年生存率的关系,并探讨P-gp、MRP1、GST-π与新辅助化疗疗效的关系。方法: 1)收集南昌大学第一附属医院诊治的口腔鳞癌石蜡标本77例(有效组50例,无效组27例),随访5年生存时间,Envision法检测P-gp、MRP1、GST-π表达水平;2)收集本单位口腔鳞癌新鲜标本33例(有效组18例,无效组15例),qPCR、WesternBlot法检测P-gp、MRP1、GST-π表达水平。结果: 1)Kaplan-Meier显示:有效组5年生存率高于无效组(P<0.05)。2)Envision、qPCR 、WesternBlot显示:无效组化疗后P -gp、MRP1表达上升量较有效组有显著差异(P<0.05),而GST-π表达上升量无显著差异(P>0.05)。结论: 口腔鳞癌新辅助化疗有效组5年生存率明显高于无效组;化疗疗效与P-gp、MRP1表达相关,表达上升量越高,疗效越差
| [1] | Pan S T, Li Z L, He Z X, et al. Molecular mechanisms for tumour resistance to chemotherapy [J]. Clin Exp Pharmacol Physiol,2016,43(8)∶723-737 |
| [2] | 冷卫东,王大章,冯戈,等.肿瘤耐药蛋白在舌鳞癌中的表达及意义[J].华西口腔医学杂志,2004,22(1)∶23-25 |
| [3] | Aprile G, Fontanella C, Bonotto M, et al. Timing and extent of response in colorectal cancer: critical review of current data and implication for future trials [J]. Oncotarget,2015,6(30)∶28716-28730 |
| [4] | 邱蔚六,薛晓帆,刘瑷如.口腔颌面部鳞癌术前辅助化疗的评价[J].华西口腔医学杂志,1991,9(3)∶167-173 |
| [5] | Sun W, Zhang X, Ding X, et al. Lactate dehydrogenase B is associated with the response to neoadjuvant chemotherapy in oral squamous cell carcinoma [J]. PLoS One,2015,10(5)∶e125976 |
| [6] | Zhu H, Liu Z, Tang L, etal. Reversal of P-gp and MRP1-mediatedmultidrug resistance by H6,agypenosideagly conform Gynostemmapentaphyllum,in vincristine-resistant human oral cancer(KB/VCR) cells [J]. Eur J Pharmacol,2012,696(1-3)∶43-53 |
| [7] | 邱嘉旋,魏军水,朱声荣,等.Ki-67、EGFR、p53及RARβ在口腔鳞状细胞癌化疗前后的变化及临床意义评价[J].口腔医学研究,2011,27(2)∶127-131 |
| [8] | Perez-Sayans M, Somoza-Martin J M, Barros-Angueira F, et al. Multidrug resistance in oral squamous cell carcinoma: The role of vacuolar ATPases [J]. Cancer Lett,2010,295(2)∶135-143 |
| [9] | Sano D, Matsuda H, Ishiguro Y, et al. Antitumor effects of IDN5109 on head and neck squamous cell carcinoma [J]. Oncol Rep,2006,15(2)∶329-334 |
| [10] | 杨锋.术前诱导化疗、术后辅助化疗对晚期舌癌患者生存情况影响的对比研究[D].广西医科大学,2010 |
| [11] | Rosario L A, O'Brien M L, Henderson C J, et al. Cellular response to a glutathione S-transferase P1-1 activated prodrug [J]. Mol Pharmacol,2000,58(1)∶167-174 |
| [12] | 黄海燕,杨蕾,李秋梨,等.诱导化疗在局部中晚期口腔鳞癌序贯治疗中功能保留作用的研究[J].中华临床医师杂志,2012,15(6)∶4305-4309 |
| [13] | Peng B, Yi S, Gu Y, et al. Purification and biochemical characterization of a novel protein-tongue cancer chemotherapy resistance-associated protein1 (TCRP1) [J]. Protein Expr Purif,2012,82(2)∶360-367 |
| [14] | Pan S T, Qin Y, Zhou Z W, et al. Plumbagin induces G2/M arrest, apoptosis, and autophagy via p38 MAPK- and PI3K/Akt/mTOR-mediated pathways in human tongue squamous cell carcinoma cells [J]. Drug Des Devel Ther,2015,9∶1601-1626 |
