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- 2017
miR-30d在舌鳞状细胞癌组织中表达及反义miR-30d对细胞增殖、侵袭能力的影响
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Abstract:
摘要 目的: 探讨miR-30d在舌鳞状细胞癌组织中表达及反义miR-30d对细胞增殖、侵袭能力的影响。方法: 选取2013年3月~2015年4月在我院行根治性手术治疗的TSCC患者68例,实时荧光定量PCR检测TSCC组织和癌旁正常组织中miR-30d基因表达,培养舌癌SCC-4细胞,分为ASO-miR-30d组、ASO-阴性对照组和空白对照组,检测各组细胞中miR-30d基因表达,MTT法检测各组细胞增殖能力,Transwell法检测各组细胞迁移和侵袭能力。结果: TSCC组织中miR-30d相对表达量(1.89±0.21),显著高于癌旁正常组织的(1.31±0.16),差异有统计学意义(P=0.000);TSCC组织中miR-30d相对表达量与TNM分期、分化程度和颈淋巴结转移有关(P<0.05);与空白对照组和ASO-阴性对照组比较,ASO-miR-30d组细胞中miR-30d相对表达量降低,差异有统计学意义(P<0.05);MTT实验结果显示,与空白对照组和ASO-阴性对照组比较,ASO-miR-30d组24 h、48 h、72 h和96 h时细胞A值均降低,差异均有统计学意义(P<0.05);与空白对照组和ASO-阴性对照组比较,ASO-miR-30d组迁移细胞数和侵袭细胞数均减少,差异均有统计学意义(P<0.05)。结论: miR-30d在TSCC组织中呈高表达,反义miR-30d可有效减少TSCC细胞增殖,抑制细胞迁移和侵袭能力
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