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- 2017
小鼠牙髓干细胞同种异体移植后体内免疫反应初探
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Abstract:
摘要 目的:初步探究小鼠牙髓干细胞同种异体移植后的全身免疫反应。方法:牙髓干细胞分离获得自C57BL/6 小鼠牙髓组织,培养至第3 代,与纳米羟基磷灰石(n-HA)复合培养形成复合物,扫描电镜观察其生长黏附情况。40只4周龄的C57BL/6 小鼠随机分成两组。实验组将复合物移植入上背部皮下,对照组将纳米羟基磷灰石植入上背部皮下,术后均使用外科方法严密缝合创口。两组动物术后均观察4周、6周处死,分离脾脏,制成脾淋巴细胞悬液,流式细胞仪测定CD4+、CD8+的值。结果:牙髓干细胞在体外与纳米羟基磷灰石结合良好;免疫组化染色实验组4周、6周均可见牙本质涎蛋白(dentin sialoprotein,DSP)阳性表达;实验组与对照组相比,术后4周、6周CD4+/CD8+值均无明显差异。结论:牙髓干细胞同种异体移植后,尚不能够引起机体的免疫排斥反应,其在体内仍具有低免疫原性,有望成为组织工程同种异体种子细胞来源
| [1] | Zhu Q, Gibson MP, Liu Q, et al. Proteolytic processing of dentin sialophospho- -protein (DSPP) is essential to dentinogenesis [J]. Biol Chem,2012,287(36))∶30426-30435 |
| [2] | Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J]. Proc Natl Acad Sci U S A, 2000, 97(25)∶13625- 13630 |
| [3] | Ding G, Niu J, Liu Y. Dental pulp stem cells suppress the proliferation of lymphocytes via transforming growth factor-β1 [J]. Hum Cell,2011,28(2))∶81-90 |
| [4] | 何维.医学免疫学[M].第2版.北京:人民卫生出版社,2010)∶22 |
| [5] | Deans RJ, Moseley AB. Mesenchymal stem cells: biology and potential clinical uses [J]. Exp Hematol, 2000, 28(8))∶875-884 |
| [6] | 聂姗姗,刘佳,张瑞涵,等.牙髓干细胞MHC分子表达与体外混合淋巴细胞的增殖[J].中国组织工程研究,2014,(50))∶8162-8167 |
| [7] | 铁晓敏,刘佳,王丽萍,等.牙髓干细胞修复再生同质异体大鼠牙髓牙本质复合体的研究[J].口腔医学研究,2015,31(7)∶645-648 |
| [8] | Masataka Y, Norimasa T, Tadao T, et al. Osteogenic effect of hyaluronic acid sodium salt in the pores of a hydroxyapatite scaffold [J]. Materials Sci Engin, 2007, 27(2))∶220-226 |
| [9] | 马路平,钟良军,张源明,等.小型猪牙周膜干细胞在羟基磷灰石支架材料上的生长[J].中国组织工程研究,2013,(29))∶5290-5295 |
| [10] | Harada H, Kettunen P, Jung HS, et al.Localization of putative stem cells in dental epithelium and their association with Notch and FGF signaling [J]. Cell Biol, 1999 ,147(1))∶105-120 |
| [11] | Fonteneau JF, Larsson M, Somersan S, et a1.Generation of high quantities of viral and tumor-specific human CD4+ and CD8+ T-cell clones using peptide pulsed mature dendritic cells [J]. Immunol Methods, 2001, 258(1-2)∶111-126 |
| [12] | Tang R,Ding G. Swine dental pulp stem cells inhibit T-cell proliferation [J]. Transplant Proc,2011,43(10))∶3955-3959 |
| [13] | Niu LL, Zheng M, Cao F, et al. Migration and differentiation of exogenous rat mesenchymal stem cells engrafted into normal and injured hearts of rats [J]. Zhonghua Yi Xue Za Zhi, 2004,84(1))∶38-42 |
| [14] | Coppe C, Zhang Y, Den Besten PK. Characterization of primary dental pulp cells in vitro [J]. Pediatr Dent,2009,31(7))∶467-471 |
| [15] | 杨雪超.CD146在人牙髓干细胞中表达的研究[J].口腔医学研究,2016,32(2))∶122-125 |
| [16] | Karamzadeh R, Eslaminejad MB, Aflatoonian R. Isolation, characterization and comparative differentiation of human dental pulp stem cells derived from permanent teeth by using two different method [J]. Vis Exp,2012 ,(69))∶4372 |
| [17] | Yang X, Zhang W, van den Dolder J, et al. Multilineage potential of STRO-1+ rat dental pulp cells in vitro [J]. Tissue Eng Regen Med,2007,1(2))∶128-35 |
| [18] | 王冉旭,潘爽,何丽娜,等.人牙髓干细胞在不同HA含量的pLGA/HA复合支架材料上的粘附研究[J].口腔医学研究,2015,31(12)∶1171-1173 |
