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- 2017
MicroRNA 34a-5p对成骨细胞炎症反应及分化的影响
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Abstract:
摘要 目的:探究microRNA 34a-5p(miR-34a-5p)对成骨细胞炎症反应及分化的调节作用,并初步探究其相关机制。方法:脂质体法瞬时转染人成骨样细胞系MG63使细胞中miR-34a-5p表达增强,同时设通用阴性对照(NC)组。牙髓卟啉单胞菌(Porphyromonas endodontalis,P.e)来源的不同浓度脂多糖(lipopolysaccharide,LPS)作用于MG63后,检测细胞内miR-34a-5p表达; 取20 mg/L P.e-LPS作用24 h后,检测细胞内IL-6表达。成骨诱导48 h后,检测细胞中成骨分化标志物以及Notch1受体的表达。结果:P.e-LPS作用于MG63细胞后,miR-34a-5p表达被抑制(P<0.05)。 MiR-34a-5p转染细胞后,与NC组相比,细胞内IL-6表达减少(P<0.01);20 mg/L P.e-LPS作用下,miR-34a-5p组IL-6表达较NC组下降更为明显(P<0.01)。成骨细胞诱导分化48 h后,miR-34a-5p组中分化标志物表达较NC对照组均显著增加(P<0.05),而Notch1受体表达被抑制(P<0.05)。结论:MiR-34a-5p可能通过Notch1信号通路,发挥抑制成骨细胞炎症和促进成骨分化的作用
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