|
|
- 2016
牙髓炎不同阶段P2X受体家族在大鼠脑干中的表达变化研究
|
Abstract:
摘要 目的:研究牙髓炎不同阶段P2X受体家族在大鼠脑干中的表达变化。方法::本实验在大鼠左上颌第一磨牙颌面开髓,并暴露于口腔菌群环境,诱导牙髓炎症。取牙髓炎症第1天、第3天、第7天和第28天时,大鼠双侧脑干的三叉神经脊束核尾端亚核(Vc)和丘脑的腹后中核(VPM),采用免疫组化和免疫荧光方法观察P2X受体家族(P2X1-7受体)的表达模式。结果:实验观察到牙髓炎症第1、3、7天显示急性牙髓炎症的组织学表现,其中第3天最为典型,而第28天显示牙髓坏死和慢性根尖周炎的组织学表现;P2X1-6受体在Vc和VPM中均表达在神经元的胞浆中,而P2X7表达在Vc和VPM中在小胶质细胞中;P2X2,P2X4和P2X7受体在急性牙髓炎症期在同侧Vc中的表达增加;P2X1-5和P2X7的表达都在双侧VPM中均上升,开髓3 d时,对侧VPM中P2X1-5和P2X7的平均表达面积显著高于同侧。结论:除P2X6受体持续高表达外,P2X1-5和P2X7受体均可受到牙髓炎症的诱导,在Vc和VPM中表达增高
| [1] | Takeda M, Kitagawa J, Nasu M, et al. Glial cell line-derived neurotrophic factor acutely modulates the excitability of rat small-diameter trigeminal ganglion neurons innervating facial skin. Brain Behav Immun, 2010, 24(1)∶72-82 |
| [2] | Balkowiec-Iskra E, Vermehren-Schmaedick A, Balkowiec A. Tumor necrosis factor-alpha increases brain-derived neurotrophic factor expression in trigeminal ganglion neurons in an activity-dependent manner. Neuroscience, 2011, 180∶322-333 |
| [3] | Takeda M, Takahashi M, Matsumoto S. Inflammation enhanced brain-derived neurotrophic factor-induced suppression of the voltage-gated potassium currents in small-diameter trigeminal ganglion neurons projecting to the trigeminal nucleus interpolaris/caudalis transition zone. Neuroscience, 2014, 26(2)∶223-231 |
| [4] | Kurata S, Goto T, K Gunjigake K, et al. Nerve Growth Factor Involves Mutual Interaction between Neurons and Satellite Glial Cells in the Rat Trigeminal Ganglion. Acta Histochem Cytochem, 2013, 46(2)∶65-73 |
| [5] | Hayasaki H, Sohma Y, Kanbara K, et al. Heterogenous GABA(B) receptor-mediated pathways are involved in the local GABAergic system of the rat trigeminal ganglion: possible involvement of KCTD proteins. Neuroscience, 2012, 218(2)∶344-358 |
| [6] | Chattipakorn SC, Sigurdsson A, Light AR, et al. Trigeminal c-Fos expression and behavioral responses to pulpal inflammation in ferrets [J]. Pain, 2002, 99(1)∶61-69 |
| [7] | Weigelt A, Terekhin P, Kemppainen P, et al. The representation of experimental tooth pain from upper and lower jaws in the human trigeminal pathway[J]. Pain, 2010, 149(3): 529-538 |
| [8] | Ford AP. In pursuit of P2X3 antagonists: novel therapeutics for chronic pain and afferent sensitization. Purinergic Signal, 2011,8(1)∶3-26 |
| [9] | Mo G, Grant R, O'Donnell D, Ragsdale DS, et al. Neuropathic Nav1.3-mediated sensitization to P2X activation is regulated by protein kinase C[J]. Mol Pain, 2011, 7(1)∶14-18 |
| [10] | Inoue K. The function of microglia through purinergic receptors: neuropathic pain and cytokine release[J]. Pharmacol Ther, 2006, 109(1-2)∶210-226 |
| [11] | Jain N, Gupta A, N M. An Insight Into Neurophysiology of Pulpal Pain: Facts and Hypotheses[J]. Korean J Pain, 2013, 26(4)∶347-355 |
| [12] | Abbracchio MP, Burnstock G, Verkhratsky A, et al. Purinergic signalling in the nervous system: an overview [J]. Trends Neurosci, 2009, 32(1)∶19-29 |
