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-  2016 

植酸作为螯合剂去除根管玷污层的效力及其对牙本质化学组成的影响
Effect of Phytic Acid Used as Chelating Agent on Smear Layer and Chemical Composition of Dentin.

DOI: 10.13701/j.cnki.kqyxyj.2016.04.014

Keywords: 植酸,牙本质,玷污层,扫描电镜,红外光谱,

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Abstract:

摘要 目的:通过体外实验评价植酸(IP6)作为根管螯合剂去除次氯酸钠溶液处理的牙本质表面玷污层的效力,探究其对次氯酸钠溶液处理的牙本质化学组成的影响。方法:24个完整无龋正畸牙取得牙本质样本随机分成4组。A组:2 mL去离子水(pH7.4)处理5 min;B组:2 mL 5%NaClO溶液(pH12)处理5 min;C组:2 mL 5%NaClO溶液处理5 min后,2 mL 17% EDTA溶液(pH7.5)处理1 min;D组:2 mL 5% NaClO溶液处理5 min后,2 mL 1% IP6溶液(pH1.3)处理1 min。SEM观察样本表面玷污层去除情况,衰减全反射红外光谱仪(ATR-IR)测量样本红外光谱,得到处理前后的M:M(matrix:mineral)和C:M(carbonate:mineral)。以双因素重复测量方差分析比较差异。结果:SEM显示处理后A组仍有大量玷污层剩余,B组玷污层去除效果不明显,C组玷污层基本完全去除,D组玷污去除效果更加明显。ATR-IR结果经统计分析后发现,A组M:M处理前后无显著差异(P=0.744),B、C、D组均显著下降(P<0.01);处理后B、C、D组明显低于A组(P<0.01),而该3组间无显著差异(P>0.05);四组C:M处理后均略低于处理前(P<0.01)。结论:植酸能够有效地去除经次氯酸钠溶液处理后的牙本质表面的玷污层,并且其与EDTA对牙本质本身化学组成的影响无显著差别

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