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- 2018
PTH-FC对大鼠骨髓间充质干细胞增殖及骨向分化的影响
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Abstract:
摘要 目的:评价甲状旁腺激素融合蛋白PTH-FC(parathyroid hormone -Fc)对大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)骨向分化的作用,筛选出PTH-FC诱导BMSCs最佳的作用时间。方法:采用全骨髓贴壁法分离培养大鼠BMSCs并进行鉴定,以1.0×10-9 mol/L PTH-FC成骨诱导液诱导BMSCs作用6 h(间歇性给药组)和48 h(持续性给药组)通过噻唑蓝(methylthiazolyldiphenyl-tetrazolium bromide, MTT)、碱性磷酸酶(alkaline phosphatase,ALP)、茜素红染色观察PTH-FC对BMSCs增殖和骨向分化的作用。结果:含1.0×10-9 mol/L PTH-FC的成骨培养液作用6 h间歇性给药组与其他组比较,ALP和茜素红染色结果显著高于其他组;MTT结果显示各实验组间比较差异无统计学意义。结论:1.0×10-9 mol/L PTH-FC作用BMSCs 6h的间歇性给药组具有最佳的骨向诱导能力,这提示PTH-FC作为骨诱导生长因子联合BMSCs对骨组织缺损的修复是可行的
| [1] | Kostenuik PJ, Ferrari S, Pierroz D, et al. Infrequent delivery of a long-acting PTH-Fc fusion protein has potent anabolic effects on cortical and cancellous bone [J]. J Bone Miner Res, 2007, 22(10):1534-1547 |
| [2] | Saidak Z, Le Henaff C, Azzi S, et al. Low-dose PTH increases osteoblast activity via decreased Mef2c/Sost in senescent osteopenic mice [J]. J Endocrinol, 2014, 223(1):25-33 |
| [3] | Liu Q, Wan Q, Yang R, et al. Effects of intermittent versus continuous parathyroid hormone administration on condylar chondrocyte proliferation and differentiation [J]. Biochim Biophys Res Commun, 2012, 424(1):182-188 |
| [4] | 黄江鸿,杨雷,廖福朋,等.低氧诱导因子-1α诱导分化的骨髓间充质干细胞复合纳米人工骨修复兔桡骨缺损[J].骨科,2016,7(3):201-206 |
| [5] | 张梅, 邬敏辰, 金坚.PTH融合蛋白研究进展[J].生物技术通报,2009,(3):25-28 |
| [6] | Psaltis PJ, Zannettino AC, Worthley SG, et al. Concise review: mesenchymal stromal cells: potential for cardiovascular repair [J]. Stem Cells, 2010, 26(9):2201-2210 |
| [7] | Gallieni M, Cucciniello E, D'Amaro E, et al. Calcium, phosphate, and PTH levels in the hemodialysis population: a multicenter study [J]. J Nephrol, 2002, 15(2):165-170 |
| [8] | 徐威,贝朝涌,粟谋,等.大鼠骨髓基质干细胞的体外培养及诱导分化[J].中国现代医生,2015,53(32):30-34 |
| [9] | Silva BC, Costa AG, Cusano NE, et al. Catabolic and anabolic actions of parathyroid hormone on the skeleton [J]. J Endocrinol Invest, 2015, 22(10):41-50 |
| [10] | Silva BC, Bilezikian JP. Chapter 15-anabolic and catabolic pathways of parathyroid hormone on the skeleton [J]. Parathyroids, 2015:233-244 |
| [11] | Chen B, Tao L, Yang X, et al. Intermittent parathyroid hormone (1-34) application regulates cAMP-response element binding protein activity to promote the proliferation and osteogenic differentiation of bone mesenchymal stromal cells, via the cAMP/PKA signaling pathway [J]. Exp Ther Med, 2016, 11(6):2399-2406 |
| [12] | Xiao Y, Shobha M, Ross C. Clonal characterization of bone marrow derived stem cells and their application for bone regeneration [J]. Int J Oral Sci, 2010, 2(3):127-135 |
| [13] | Kuo SW, Rimando MG, Liu YS, et al. Intermittent administration of parathyroid hormone 1-34 enhances osteogenesis of human mesenchymal stem cells by regulating protein kinase Cδ [J]. Int J Mol Sci, 2017, 18(10):2221 |
| [14] | Liu X, Liao X, Luo E, et al. Mesenchymal stem cells systemically injected into femoral marrow of dogs home to mandibular defects to enhance new bone formation [J]. Tissue Eng Part A,2014,20(3-4):883 |
| [15] | Mannstadt M, Jüppner H, Gardella TJ. Receptors for PTH and PTHrP: their biological importance and functional properties [J]. Am Physiol, 1999, 277(2):665-675 |
| [16] | Bosnakovski D, Mizuno M, Kim G, et al. Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells [J]. Cell Tissue Res, 2005, 319(2):243-253 |
| [17] | Encina NR, Billotte WG, Hofmann MC. Immunomagnetic isolation of osteoprogenitors from human bone marrow stroma [J]. Lab Invest,1999, 79(4):449 |
| [18] | Reinshagen H, Auw-Haedrich C, Sorg RV, et al. Corneal surface reconstruction using adult mesenchymal stem cells in experimental limbal stem cell deficiency in rabbits [J]. Acta Ophthalmol, 2011, 89(8):741-748 |
| [19] | Wu W, Le AV, Mendez JJ, et al. Osteogenic performance of donor-matched human adipose and bone marrow mesenchymal cells under dynamic culture [J]. Tissue Eng Part A, 2015, 21(9-10):1621-1632 |
