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- 2017
载锌多壁碳纳米管/壳聚糖复合材料成骨性能的研究
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Abstract:
摘要 目的:探究载锌多壁碳纳米管/壳聚糖复合材料上的锌离子是否有促进成骨细胞增殖和分化的作用,以及该材料上锌离子的适宜浓度。方法:制备载有不同含量锌离子的多壁碳纳米管/壳聚糖复合材料,并用扫描电镜观察。其中,0.2%(质量百分数)锌含量的复合材料为低锌组,1%(质量百分数)锌含量的复合材料为中锌组,2.5%(质量百分数)锌含量的复合材料为高锌组,不含锌的复合材料为对照组。将MC3T3-E1细胞接种于4种材料表面进行培养,分别用MTT法、碱性磷酸酶试剂盒、ELISA法、激光共聚焦显微镜检测MC3T3-E1细胞在复合材料上的增殖量、碱性磷酸酶活性、骨钙素水平、细胞形态。结果:扫描电镜观察到该材料具有大量孔隙结构;mtt检测中24 h、48 h、72 h时,中锌组细胞增值量高于对照组(P<0.05),低锌组细胞增值量与对照组无明显差距,高锌组细胞增值量小于对照组(P<0.05);碱性磷酸酶活性检测中24 h、48 h、72 h时,中锌组高于对照组(P<0.05),低锌组与对照组无明显差距,高锌组小于对照组(P<0.05);骨钙素水平检测中48 h时,中锌组骨钙素浓度高于对照组(P<0.05),低锌组骨钙素浓度与对照组无明显差距,高锌组骨钙素浓度小于对照组(P<0.05);激光共聚焦观察到,在细胞接种48 h后,中锌组细胞伸展情况最好,低锌组细胞伸展情况较中锌组差,高锌组细胞伸展较差并且有部分细胞皱缩,对照组细胞伸展情况与低锌组无明显差异。结论:中锌组复合材料更有利于MC3T3-E1细胞的增殖和分化
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