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- 2015
模拟微重力对人牙髓干细胞在裸鼠体内矿化能力的影响
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Abstract:
摘要 目的:研究模拟微重力环境对聚乳酸-羟基乙酸(PLGA)支架上的人牙髓干细胞(human dental pulp stem cells, hDPSCs)在裸鼠体内矿化能力的影响。方法:分离、培养hDPSCs并进行鉴定,然后将hDPSCs接种到PLGA支架上培养72 h后随机分为两组,普通重力组和模拟微重力组,经矿化诱导液培养1周后分别将细胞支架复合物移植到裸鼠体内,植入术后4周取材,进行组织学(HE和Vonkossa)和免疫组织化学(DSPP)检测。结果:HE染色均可见有血管生成,Vonkossa染色均为阳性,模拟微重力组DSPP的表达水平明显高于普通重力组(P<0.05)。结论:模拟微重力有利于hDPSCs在裸鼠体内的矿化
| [1] | Gronthos S, Brahim J, Li W, et al. Stem cell properties of human dental pulp stem cells [J]. Dent Res, 2002, 81(8)∶531e5 |
| [2] | Ma PX. Biomimetic materials for tissue engineering [J]. Adv Drug Deliv Rev, 2008, 60(2)∶184e98 |
| [3] | Shi Li, Zhaofeng Ma, Zhongying Niu, et al. NASA-Approved Rotary Bioreactor Enhances Proliferation and Osteogenesis of Human Peridontal Ligament Stem Cells [J]. Stem Cells Dev, 2009, 18(9)∶1273-1278 |
| [4] | Facer SR, Zaharias RS, Andracki ME, et al. Rotary culture enhances pre-osteoblast aggregation and mineralization [J]. Dent Res, 2005, 84∶542-547 |
| [5] | Hammond T G, Hammond J M. Optimized suspension culture: the rotating-wall vessel [J]. American Journal of Physiology, 2001, 281∶F12-25 |
| [6] | 费晓磊,李艳萍,潘爽,等.模拟微重力对人牙髓干细胞-PLGA复合物矿化的影响[J].口腔医学研究,2013,29(2)∶135-137 |
| [7] | 张巍巍,李艳萍,梁鹏,等.人牙髓干细胞在聚乳酸-聚乙醇酸共聚物支架上粘附与增殖的研究[J].口腔医学研究,2011,27(12)∶1049-1052 |
| [8] | Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J]. Proc Natl Acad Sci USA, 2000, 97(25)∶13625e30 |
| [9] | Dai ZQ, Wang R, Ling SK, et al. Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells [J]. Cell Prolif, 2007, 40∶671-684 |
| [10] | Dima Sheyn, Gadi Pelled, Dvir Netanely, et al. The effect of simulated microgravity on human mesenchymal stem cells cultured in an osteogenic differentiation system: a bioinformatics study [J]. Tissue Eng: Part A, 2010, 16(11)∶3403-3412 |
| [11] | Qin C, Brunn JC, Cadena E, et al. Dentin sialoprotein in bone and dentin sialophosphoprotein gene expressed by osteoblasts [J]. Connective Tissue Research, 2003, 44∶179-183 |
