|
|
- 2017
微阵列芯片分析成牙骨质细胞矿化过程中microRNA表达谱的差异
|
Abstract:
摘要 目的:诱导小鼠成牙骨质细胞矿化,研究在成牙骨质细胞矿化过程中microRNA表达谱的变化。方法:体外培养小鼠成牙骨质细胞系OCCM30,使用抗坏血酸、β-甘油磷酸钠和地塞米松诱导成牙骨质细胞矿化。实时荧光定量PCR(qPCR)检测碱性磷酸酶和骨钙蛋白表达量变化来验证矿化情况。应用miRNA microarray 芯片技术,分析成牙骨质细胞矿化过程中miRNA表达谱的变化。QPCR对芯片结果进行验证。结果:成功诱导了成牙骨质细胞的矿化。MiRNA microarray 芯片检测发现成牙骨质细胞矿化过程中miRNA表达谱出现明显改变,其中上调的有4个,下调的有39个。QPCR证明芯片结果准确可靠。结论:建立了小鼠成牙骨质细胞矿化的细胞模型,应用miRNA芯片发现小鼠成牙骨质细胞矿化过程中差异表达的miRNA,提示这些miRNA可能参与成牙骨质细胞矿化过程的调控
| [1] | Bosshardt, D.D.,K.A. Selvig,Dental cementum: the dynamic tissue covering of the root [J]. Periodontol 2000,1997,13(1)∶41-75 |
| [2] | Zeichner-David, M.,Regeneration of periodontal tissues: cementogenesis revisited [J]. Periodontol 2000,2006,41(2)∶196-217 |
| [3] | Li, J.,X. He,W. Wei, et al. MicroRNA-194 promotes osteoblast differentiation via downregulating STAT1 [J]. Biochem Biophys Res Commun,2015,460(2)∶482-8 |
| [4] | Cao, Z.,H. Zhang,X. Zhou, et al. Genetic evidence for the vital function of Osterix in cementogenesis [J]. J Bone Miner Res,2012,27(5)∶1080-92 |
| [5] | Bartel, D.P.,MicroRNAs: genomics, biogenesis, mechanism, and function [J]. Cell,2004,116(2)∶281-97 |
| [6] | Huang, C.,J. Geng,S. Jiang,MicroRNAs in regulation of osteogenic differentiation of mesenchymal stem cells [J]. Cell Tissue Res,2016:[Epub ahead of print] |
| [7] | 邱雨,任嫒姝,庞琴,等.信号素3A对成牙骨质细胞系OCCM-30增殖和矿化功能影响的实验研究[J].口腔医学研究,2016,32(5)∶445-448 |
| [8] | 蔡洁明,舒传继,杨伟民,等.miRNA-31和STAT-3在舌鳞状细胞癌中的表达及临床意义[J].口腔医学研究,2015,31(8)∶820-823 |
| [9] | Grzesik, W.J.,A.S. Narayanan,Cementum and periodontal wound healing and regeneration [J]. Crit Rev Oral Biol Med,2002,13(6)∶474-84 |
| [10] | Yu, S.,Q. Geng,Q. Pan, et al. MiR-690, a Runx2-targeted miRNA, regulates osteogenic differentiation of C2C12 myogenic progenitor cells by targeting NF-kappaB p65 [J]. Cell Biosci,2016,6(1)∶10-16 |
| [11] | Huang, C.,J. Geng,X. Wei, et al. MiR-144-3p regulates osteogenic differentiation and proliferation of murine mesenchymal stem cells by specifically targeting Smad4 [J]. FEBS Lett,2016,590(6)∶795-807 |
| [12] | D'Errico, J.A.,R.L. MacNeil,T. Takata ,et al. Expression of bone associated markers by tooth root lining cells, in situ and in vitro [J]. Bone,1997,20(2)∶117-26 |
| [13] | 胡芝爱,潘洁,李煜昱,等.DAPT对成牙骨质细胞增殖活性的影响[J].口腔医学研究,2016,32(2)∶117-121 |
| [14] | 王鑫,王姗,孟琰,等.NSCL-P患者中异常表达microRNA与WNT家族的关联性研究[J].口腔医学研究,2016,32(5)∶470-473 |
| [15] | Cao, Z.,R. Liu,H. Zhang, et al. Osterix controls cementoblast differentiation through downregulation of Wnt-signaling via enhancing DKK1 expression [J]. Int J Biol Sci,2015,11(3)∶335-44 |
| [16] | Bosshardt, D.D. Are cementoblasts a subpopulation of osteoblasts or a unique phenotype [J]. J Dent Res,2005,84(5)∶390-406 |
| [17] | 蒋少云,薛栋,邓嘉胤.小分子RNA在干细胞成骨及成软骨分化中的作用[J].口腔医学研究,2015,31(8)∶845-847 |
