|
|
- 2017
IGF-1通过PI3K/AKT信号通路促进牙髓细胞增殖及碱性磷酸酶活性的研究
|
Abstract:
摘要 目的:研究IGF-1对牙髓细胞增殖和碱性磷酸酶(ALP)活性及PI3K/AKT信号通路的影响。方法:采用酶消化法分离培养原代人牙髓细胞。Western blot检测牙髓组织中IGF-1蛋白表达,不同浓度的IGF-1处理牙髓细胞7d,CCK8法检测细胞增殖。用IGF-1(100 μg/L)及LY294002(10 μmol/L)分别单独或同时处理牙髓细胞,培养7 d后MTT实验检测细胞增殖;在培养第3、5、7、14天时检测细胞ALP 活性;在培养第7天时用Western blot检测AKT和p-AKT蛋白表达情况。结果:IGF-1在牙髓炎组织中低表达, IGF-1在20~100 μg/L浓度范围内从作用第3天开始能够显著促进牙髓细胞增殖(P<0.05或P<0.01),且具有剂量和时间依赖效应。LY294002能够抑制牙髓细胞的增殖和碱性磷酸酶活性,具有时间依赖性。IGF-1能促进p-AKT蛋白表达,而 LY294002能减低IGF-1对p-AKT蛋白表达的促进作用。结论:IGF-1可以促进牙髓细胞增殖和碱性磷酸酶活性,且具有浓度和时间依赖性,作用机制可能与PI3K/AKT信号通路相关。 [关键词] 牙髓细胞 细胞增殖 碱性磷酸
| [1] | Tapia O, Riquelme I, Leal P, et al. The PI3K/AKT/mTOR pathway is activated in gastric cancer with potential prognostic and predictive significance [J]. Virchows Archiv, 2014, 465(1)∶25-33 |
| [2] | 王雪鹏,李茂强,边振宇,等.PI3 K/Akt信号通路在骨髓间充质干细胞增殖及成骨分化调控中的作用[J].中华骨质疏松和骨矿盐疾病杂志,2014,7(3)∶250-257 |
| [3] | Kadri Z, Lefevre C, Goupille O, et al. Erythropoietin and IGF-1 signaling synchronize cell proliferation and maturation during erythropoiesis [J]. Genes & development, 2015, 29(24)∶2603-2616 |
| [4] | 关祥艳,李启梦,刘惠娟,等.PI3K/AKT信号通路对体外人牙髓细胞增殖和成牙本质向分化能力的影响[J].中华口腔医学研究杂志:电子版,2014,8(3)∶10-13 |
| [5] | Ren J G, Seth P, Clish C B, et al. Knockdown of Malic Enzyme 2 Suppresses Lung Tumor Growth, Induces Differentiation and Impacts PI3K/AKT Signaling [J]. Scientific Reports, 2014, 4(4)∶5414-5414 |
| [6] | 罗裕旋,吴传安,杨伟康,等.骨碱性磷酸酶 N-端骨钙素^ 99Tc^ m-亚甲基二膦酸盐骨显像与早期骨代谢改变[J].山西医药杂志, 2015, 44(24)∶2859-2861 |
| [7] | Aslantas E E, Buzoglu H D, Karapinar S P, et al. Age-related Changes in the Alkaline Phosphatase Activity of Healthy and Inflamed Human Dental Pulp [J]. Journal of endodontics, 2016, 42(1)∶131-134 |
| [8] | 王雪.IGF-1通过JAK-STAT通路促进牙髓细胞增殖和分化的体外研究[D].河北医科大学,2015 |
| [9] | Chang M C, Hung H P, Lin L D, et al. Effect of interleukin-1β on ICAM-1 expression of dental pulp cells: role of PI3K/Akt, MEK/ERK, and cyclooxygenase [J]. Clinical oral investigations, 2015, 19(1)∶117-126 |
| [10] | Chen J, Duan Y, Zhang X, et al. Genistein induces apoptosis by the inactivation of the IGF-1R/p-Akt signaling pathway in MCF-7 human breast cancer cells [J]. Food & function, 2015, 6(3)∶995-1000 |
| [11] | 王冉旭,潘爽,何丽娜,等.人牙髓干细胞在不同 HA含量的 PLGA/HA 复合支架材料上的粘附研究[J].口腔医学研究,2015,31(12)∶1171-1173 |
| [12] | Al-Kharobi H, El-Gendy R, Devine D A, et al. The role of the insulin-like growth factor (IGF) axis in osteogenic and odontogenic differentiation [J]. Cellular and molecular life sciences, 2014, 71(8)∶1469-1476 |
| [13] | 陈巍.胰岛素样生长因子-1和转化生长因子-β1在来曲唑诱导多囊卵巢综合征模型大鼠中的表达及意义[J].中国老年学杂志,2016,36(14)∶3395-3397 |
