|
|
- 2017
成年牛及胎牛β-磷酸三钙煅烧骨骨块的制备、表征和细胞相容性研究
|
Abstract:
摘要 目的:对比研究焦磷酸钠(Na4P2O7.10H2O,NP)和磷酸二氢铵(NH4H2PO4,MAP)制备的成年牛及胎牛β-TCP煅烧骨骨块的表征及细胞相容性,以期制备出一种理化性能较好且生物安全性可靠的纯相β-TCP煅烧骨骨块。方法:以XRD衍射分析、EDX能谱分析、电镜扫描、Micro-CT扫描及材料力学性能检测研究煅烧骨骨块的表征,以体外细胞毒性实验及细胞与材料的复合实验探究煅烧骨骨块的细胞相容性。结果:NH4H2PO4制备的两种煅烧骨骨块其β-TCP纯度较高,Ca/P比值也均接近于1.47;胎牛煅烧骨骨块的孔隙率、抗压强度及抗弯曲强度均高于成年牛煅烧骨骨块(P<0.05);NH4H2PO4制备的两种煅烧骨骨块细胞毒性均为0级,但胎牛煅烧骨骨块上细胞的粘附与增殖速率高于成年牛煅烧骨骨块。结论:NH4H2PO4制备的胎牛β-TCP煅烧骨骨块具有较好的理化性能及细胞相容性,较适用于非承重区骨缺损的修复
| [1] | Akeda K, An HS, et al. Platelet-rich plasma stimulatesporcine articular chondrocyte proliferation and matrix biosynthesis [J]. Osteoarthr Cartil, 2006, 14(12)∶1272 |
| [2] | 郑启新,刘苏南,煅烧牛松质骨的制备、理化性能及生物相容性研究[J].生物医学工程学杂志,2005,22(1)∶95-98 |
| [3] | 赵铭,郑启新.异种煅烧骨材料的研究进展[J].国外医学生物医学工程册,2003,26(4)∶188-192 |
| [4] | Feng-Huei Lin, Chun-Jen Liao, Ko-Shao Chen, et al. Preparation of a biphasic porous bioceramic by heating bovinecancellous bone with Na<sub>4</sub>P<sub>2</sub>O<sub>7</sub>.10H<sub>2</sub>O addition [J]. Biomaterials, 1999, 20∶475-484 |
| [5] | 郭朝邦,金海波,杨筠,等.煅烧牛松质骨制备β-TCP/HAP/Ca2P207多孔陶瓷反应机制研究[J].稀有金属材料与工程,2010,39(6)∶1071-1074 |
| [6] | 郑启新,刘苏南,煅烧牛松质骨的制备、理化性能及生物相容性研究[J].生物医学工程学杂志,2005,22(1)∶95-98 |
| [7] | 裴赛敏.涂覆壳聚糖涂层镁基复合材料的生物相容性研究[D].中南大学,2011 |
| [8] | 葛亮,苟三怀,杨四川,等.纳米羟基磷灰石/硫酸钙复合人工骨的生物安全性研究[J].生物骨科材料与临床研究,2007,4(2)∶4-8 |
| [9] | 张玉富,王常勇,廖文,等.β-磷酸三钙多孔陶瓷与骨髓间充质干细胞生物相容性的体外实验研究[J].华中科技大学学报(医学版),2004,33(5)∶615-618 |
| [10] | Campion CR, Chander C, Buckland T, et al. Increasing strut porosity insilicate-substituted calcium-phosphate bone graft substitutes enhances osteogenesis [J]. J Biomed Mater Res B Appl Biomater, 2011, 97∶245-254 |
| [11] | Alice Cheng, Aiza Humayun, David J Cohen, et al. Additively manufactured 3D porous Ti-6Al-4V constructs mimic trabecular bone structure and regulate osteoblast proliferation, differentiation and local factor production in a porosity and surface roughness dependent manner [J]. Biofabrication,2014,6(4)∶1-13 |
| [12] | Lin FH, Liao CJ, Chen KS, et al. Preparation of beta TCP /HAP biphasic ceramics with natural bone structure by heating bovine cancellous bone with the addition of (NH<sub>4</sub>)<sub>2</sub>HPO<sub>4</sub> [J]. J Biomed MaterRes,2000,51(2)∶157-163 |
