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- 2015
骨皮质切开术辅助大鼠正畸牙移动中OPNmRNA、BSPmRNA、OCNmRNA表达的研究
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Abstract:
摘要 目的:观察骨桥蛋白(OPN),骨涎蛋白(BSP),骨钙蛋白(OCN)在大鼠骨皮质切开术辅助正畸牙移动过程中的表达情况。方法:选取健康雄性Wistar大鼠65只,60只大鼠随机分成3组:组Ⅰ:选择性骨皮质切开术;组Ⅱ:传统的牙齿移动;组Ⅲ:混合组,剩余5只作为空白对照组(不作任何处理)。用镍钛螺旋拉簧施加约60 g力近中移动上颌实验侧第一磨牙,在第一磨牙颊腭侧行骨皮质打孔术式,分别在加载第7、14、21、28、42天颈椎脱臼处死取材,进行SYBR Green实时荧光定量PCR技术,对Ct值进行分析。结果:混合组中的成骨细胞相关细胞因子:OPN(骨桥蛋白),Bone sialoprotein(BSP骨涎蛋白),Osteocalcin(OCN骨钙蛋白)的表达早期增加暗示了同化合成反应的增加。结论:正畸牙齿移动初期骨皮质切开术加速了牙齿移动,改变了早期骨代谢状态,成骨活跃,支持区域性骨代谢加速学说
| [1] | Kole H.Surgical operations of the alveolar ridge to correct occlusal abnorm-alities[J].Oral Surg Oral Med Oral Pathol,1959a,12(5)∶515-529 |
| [2] | Duker,J.Experimental animal research into segmental alveolar movement after corticotomy[J].J.Maxillofac.Surg,1975,3(2)∶81-84 |
| [3] | Suya H.Corticotomy in orthodontics: Mechanical and Biological Basics in Orthodontic Therapy.Heidelberg,Germany:Htitlig Buch Verlag,1991.PP207-226 |
| [4] | Sebaoun J.D.,Kantarci A.,Turner J.W.,et al.Modeling of trabecular bone and lamina dura following selective alveolar decortications in rats[J].J.Periodontal, 2008,79(9)∶1679-1688 |
| [5] | Kole H.Surgical operations on the alveolar ridge to correct occlusal abn-ormalities[J].Oral Surg Oral Med Oral Pathol,l959b,12(4)∶414-420 |
| [6] | Kole H.Surgical operations on the alveolar ridge to correct occlusal abn-ormalities[J].Oral Surg Oral Med Oral Pathol,1959c,12(3)∶277-288 |
| [7] | Wilcko WM,Wilcko MT,Bouquot JE,et al.Rapid orthodontics with alveolarreshaping:two case reports of decrowding[J].Int J Periodontics Restorative Dent,2001,21(1)∶9-19 |
| [8] | Frost H.M.The regional acceleratory phenomenon: A review.Henry Ford Hosp.Med.J.31∶3.1983 |
| [9] | Vignery A,Baron R.Dynamic histomorphometry of alveolar bone remodeli-ng in the adult rat[J].Anat Rec,1980,196(2)∶191-200 |
| [10] | Dolce C,Anguita C,Brinkley L.Effects of sialoadenectomy and exogenonsEGF on molar drift and orthodontic tooth movement in rat[J].Am J Physiology,1994:266(5pt1)∶E731-738 |
| [11] | King GJ,Keeling SD,McCoy EA,et al.Measuring dental drift and orthod-ontic tooth movement in response to various initial forces in adult rats[J].Am JOrthod and Deniofacial Orthop,1991,99(5)∶456-465 |
| [12] | Wilcko WM,Wilcko MT,Bouquot JE,et al.Rapid orthodontics with alveolarreshaping:two case reports of decrowding[J].Int J Periodontics Restorative Dent,2001,21(1)∶9-19 |
| [13] | Liu Z,Luyten FP,Lammens J,Dequeker J.Molecular signaling in bone fracture healing and distraction osteogenesis.HistolHistopathol, 1999,14∶587-59 |
