|
|
- 2017
小鼠涎腺发育晚期miRNA基因筛选及功能预测
|
Abstract:
摘要 目的:筛选小鼠涎腺发育晚期差异表达的miRNA,为涎腺组织工程垫定实验基础。方法: 应用生物基因芯片构建小鼠涎腺发育胚胎第18天(E18.5)、19天(E19.5)、出生后0 d(P0)、出生后3 d(P3),4个时期下颌下腺miRNA表达谱,应用GENESPRING10.0进行Quantile normalization。基于Gene Ontology数据库,预测基因的功能。结果:建立小鼠涎腺发育晚期的miRNA表达谱。其中显著变化的有:mmu-miR-133a*,mmu-miR-721,mmu-miR-1,mmu-miR-133b,mmu-miR-133a ,mmu-miR-206。结论:显著变化的miRNA在涎腺发育过程中有可能起重要作用
| [1] | Filipowicz W. RNAi: the nuts and bolts of the RISC machine [J]. Cell, 2005, 122(1)∶17-20 |
| [2] | Selbach M, Schwanhausser B, Thierfelder N, Fang Z, Khanin R, Rajewsky N. Widespread changes in protein synthesis induced by microRNAs [J]. Nature, 2008, 455(1)∶58-63 |
| [3] | Guarnieri DJ, DiLeone RJ. MicroRNAs: a new class of gene regulators [J]. Ann Med, 2008, 40(2)∶197-208 |
| [4] | Mathupala SP,Guthikonda M ,Sloan AE. RNAi based approaches to the treatment of malignant glioma [J]. Teehnol Cancer Res Treat, 2006, 5(3)∶261-269 |
| [5] | Ambros V. The evolution of our thinking about microRNAs [J]. Nat Med, 2008, 14∶1036-40 |
| [6] | Griffiths-Jones S. miRBase: The microRNA sequence data-base [J]. MethodsMol Biol, 2006, 342(1)∶129-138 |
| [7] | Farahat M, Sathi GA, Hara ES, Taketa H,Kuboki T, Matsumoto T (2017) MSCs feeder layers induce SMG self-organization and branching morphogenesis [J]. PLoS ONE 12(4)∶e0176453 |
| [8] | Hirayama M,Tsubota K,Tsuji T. Bioengineered Lacrimal Gland Organ Regeneration in Vivo [J]. Journal of Functional Biomaterials, 2015, 6(3)∶634-649 |
| [9] | Myat MM, Andrew DJ. Organ shape in the drosophila salivary stand is contzo1.1.ed by Ie lated,sequendM intemalizatlon of the primordial [J]. Development, 2000, 127(4)∶679-691 |
| [10] | Nelson PT,Baldwin DA,Kloosterman WP,et al. RAKE and LNA- ISH reveal microRNA expression and localization in archival human brain [J]. RNA, 2006, 12(2)∶187-191 |
| [11] | Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function [J]. Cell, 2004, 116(2)∶281-97 |
| [12] | Chen K, Rajewsky N. Deep conservation of microRNA-target relationships and 30UTR motifs in vertebrates, flies, and nematodes [J]. Cold Spring Harb Symp Quant Biol, 2006, 71(1)∶149-56 |
| [13] | Chartoumpekis DV, Zaravinos A, Ziros PG, et al. Differential Expression of MicroRNAs in Adipose Tissue after Long-Term High-Fat Diet-Induced Obesity in Mice [J]. PLoS ONE 2012,7(4): e34872. doi:10.1371/journal.pone.0034872 |
| [14] | Michael Molnick,Tina Jaskoll. Mouse submandibular gland morphogenesis: a paradigm for embryonic signal processing [J]. Crit Rev Oral Biol Med, 2000, 11(2)∶199-215 |
| [15] | Gresik EW. The granular convoluted tubule (GCT) cell of rodent submandibular glands [J]. Microsc Res Tech, 1994, 27(1)∶1-24 |
| [16] | Tsuda N,Ishiyama S,Li Y,et al. Synthetic microRNA designed to target glioma-associated antigen 1 transcription factor inhibits division and induces late apoptosis in pancreatic tumor cells [J]. Clin Cancer Res, 2006, 12(21)∶6557- 6564 |
| [17] | Lee S,Vasudevan S.Post-transcriptional stimulafion of gene expression by micmRNAs [J]. Advances in Experimental Medicine and Biology, 2013, 768(1)∶97-126 |
| [18] | Yang B, Lin H, Xiao J, Lu Y, Luo X, et al. The muscle-specific microRNA miR-1 regulates cardiac arrhythmogenic potential by targeting GJA1 and KCNJ2 [J]. Nat Med, 2007, 13(4)∶486-491 |
