|
|
- 2015
持续牵张促进小鼠骨髓间充质干细胞成骨向分化机制的研究
|
Abstract:
摘要 目的:研究p38MAPK在持续性机械牵张力促进C57BL/6J小鼠骨髓间充质干细胞成骨分化过程中的作用机制。方法:C57BL/6J小鼠BMSCs被随机分为对照组和牵张力阻断组。牵张力阻断组预先用p38MAPK特异性阻断剂SB203580处理1 h,后用Flexercell加力仪加载0.5Hz,0.8%的牵张力。对照组不做特殊处理。分别对两组细胞施加0、30 min和60 min的牵张力。采用Western blot检测P-p38MAPK蛋白的表达情况,Real time-PCR检测成骨基因ALP、COL I、OCN mRNA的表达变化。结果:C57BL/6J小鼠BMSCs传代后细胞生长状态稳定,呈梭形,具有多向分化潜能。Real time-PCR结果显示对照组BMSCs中ALP、COL I、OCN mRNA表达在不同时间点(30 min与0 min,60 min与30 min)间差异均有统计学意义(P<0.05);对照组ALP、COLI、OCN的表达量均明显高于同一时间点(30 min和60 min)牵张力阻断组(P<0.05)。Western blot结果显示,对照组内BMSCs中P-p38MAPK的蛋白水平在不同时间点间(30 min与0 min,60 min与30 min)差异均有统计学意义(P<0.05);对照组P-p38MAPK蛋白的表达量均明显高于同一时间点(30 min和60 min)牵张力阻断组(P<0.05)。结论:所采用的0.5 Hz,0.8%的机械牵张力在持续牵张力下可通过p38MAPK 通路促进小鼠BMSCs向成骨细胞分化
| [1] | Koike M, Shimokawa H, Kanno Z, et al. Effects of mechanical strain on proliferation and differentiation of bone marrow stromal cell line ST2 [J]. Journal of bone and mineral metabolism, 2005, 23(3)∶219-25 |
| [2] | Zhao Y, Song T, Wang W, et al. P38 and ERK1/2 MAPKs act in opposition to regulate BMP9-induced osteogenic differentiation of mesenchymal progenitor cells [J]. PloS one, 2012, 7(8)∶e43383 |
| [3] | Zhang P, Wu Y, Dai Q, et al. p38-MAPK signaling pathway is not involved in osteogenic differentiation during early response of mesenchymal stem cells to continuous mechanical strain [J]. Molecular and cellular biochemistry, 2013, 378(1-2)∶19-28 |
| [4] | Soleimani M, Nadri S. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow [J]. Nature protocols, 2009, 4(1)∶102-6 |
| [5] | Zhu H, Guo Z K, Jiang X X, et al. A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone [J]. Nature protocols, 2010, 5(3)∶550-60 |
| [6] | Suzuki A, Guicheux J, Palmer G, et al. Evidence for a role of p38 MAP kinase in expression of alkaline phosphatase during osteoblastic cell differentiation [J]. Bone, 2002, 30(1)∶91-8 |
| [7] | Wang L, Li JY, Zhang XZ, et al. Involvement of p38MAPK/NF-κB signaling pathways in osteoblasts differentiation in response to mechanical stretch [J]. Annals of biomedical engineering, 2012, 40(9)∶1884-94 |
| [8] | Du QC, Zhang DZ, Chen XJ, et al. The Effect of p38MAPK on Cyclic Stretch in Human Facial Hypertrophic Scar Fibroblast Differentiation [J]. PloS one, 2013, 8(10)∶e75635 |
| [9] | Li F, Yan X, Wang Q, et al. Effect of p38 mitogen-activated protein kinase signaling pathway during stretch-induced differentiation process of C2C12 myoblasts [J]. Hua xi kou qiang yi xue za zhi, 2012, 30(6)∶574-8 |
| [10] | Ming L, Ge B, Wang M, et al. Comparison between 8‐prenylnarigenin and narigenin concerning their activities on promotion of rat bone marrow stromal cells' osteogenic differentiation in vitro [J]. Cell proliferation, 2012, 45(6)∶508-15 |
| [11] | Ellegaard M, Kringelbach T, Syberg S, et al. The effect of PTH (1‐34) on fracture healing during different loading conditions [J]. Journal of Bone and Mineral Research, 2013, 28(10)∶2145-55 |
| [12] | Rachmiel A, Emodi O, Gutmacher Z, et al. Oral and dental restoration of wide alveolar cleft using distraction osteogenesis and temporary anchorage devices [J]. Journal of Cranio-Maxillofacial Surgery, 2013, 41(8)∶728-34 |
| [13] | Simmons C A, Matlis S, Thornton A J, et al. Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK1/2) signaling pathway [J]. Journal of biomechanics, 2003, 36(8)∶1087-96 |
