|
|
- 2017
ALK2对小鼠髁突软骨细胞增殖分化的影响
|
Abstract:
摘要 目的:探讨ALK2对小鼠髁突软骨细胞增殖及成软骨分化的作用。方法:采用酶消化法提取小鼠髁突软骨细胞并鉴定。体外采用siRNA沉默小鼠髁突软骨细胞中Alk2基因的表达,通过MTT法检测细胞增殖。通过实时定量RT-PCR方法检测软骨细胞标志基因的表达。结果:倒置显微镜观察体外培养的小鼠髁突软骨细胞形态。免疫组化染色显示COLⅠ强阳性,AGGRECAN弱阳性,COLⅡ弱阳性。Pellet培养后实时定量RT-PCR检测发现ColⅡ,ColⅩ和ColⅠ表达上调。证实所分离的细胞是髁突软骨细胞。MTT结果显示Alk2沉默后细胞数量增加,实时定量RT-PCR结果显示Alk2沉默后软骨细胞基因表达下调,并有显著统计学意义。结论:ALK2抑制小鼠髁突软骨细胞的增殖,促进小鼠髁突软骨细胞的成软骨分化
| [1] | Shen G, Darendeliler MA. The adaptive remodeling of condylar cartilage-a transition from chondrogenesis to osteogenesis [J]. Journal of dental research, 2005, 84(8)∶691-699 |
| [2] | Silbermann M, Frommer J. The nature of endochondral ossification in the mandibular condyle of the mouse [J]. The Anatomical Record, 1972, 172(4)∶659-667 |
| [3] | Culbert AL, Chakkalakal SA, Theosmy E G, et al. Alk2 regulates early chondrogenic fate in fibrodysplasia ossificans progressiva heterotopic endochondral ossification [J]. Stem Cells, 2014, 32(5)∶1289-1300 |
| [4] | Karmali PP, Chaudhuri A. Cationic liposomes as non-viral carriers of gene medicines: Resolved issues, open questions, and future promises [J]. Medicinal research reviews, 2007, 27(5)∶696-722 |
| [5] | Zhou G, Zheng Q, Engin F, et al. Dominance of SOX9 function over RUNX2 during skeletogenesis [J]. Proceedings of the National Academy of Sciences, 2006, 103(50)∶19004-19009 |
| [6] | Dudas M, Sridurongrit S, Nagy A, et al. Craniofacial defects in mice lacking BMP type I receptor Alk2 in neural crest cells [J]. Mechanisms of development, 2004, 121(2)∶173-182 |
| [7] | Balic A, Adams D, Mina M. Prx1 and Prx2 cooperatively regulate the morphogenesis of the medial region of the mandibular process [J]. Developmental Dynamics, 2009, 238(10)∶2599-2613 |
| [8] | Miyazono K, Kamiya Y, Morikawa M. Bone morphogenetic protein receptors and signal transduction [J]. Journal of biochemistry, 2010, 147(1)∶35-51 |
| [9] | Rigueur D, Brugger S, Anbarchian T, et al. The type I BMP receptor ACVR1/ALK2 is required for chondrogenesis during development [J]. Journal of Bone and Mineral Research, 2015, 30(4)∶733-741 |
| [10] | Embree MC, Kilts TM, Ono M, et al. Biglycan and fibromodulin have essential roles in regulating chondrogenesis and extracellular matrix turnover in temporomandibular joint osteoarthritis [J]. The American journal of pathology, 2010, 176(2)∶812-826 |
