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- 2017
A型钾离子通道对人舌鳞癌Tca8113细胞增殖的影响
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Abstract:
摘要 目的:探究A型钾离子(Potassium,K+)通道抑制后对舌鳞状细胞癌株(Tca8113)细胞的影响。方法:全细胞膜片钳技术记录和分析A型K+通道阻滞剂4-氨基吡啶(4-AP)对Tca8113细胞K+电流的影响;MTT检测A型K+通道对细胞增殖的作用。结果:在终浓度为2 mmol/L 4-AP作用下,A型K+电流的峰值从(267.04±13.84) pA降到(124.81 ±5.24) pA。在一定范围内,不同浓度4-AP能抑制Tca8113细胞在体外的增殖,且抑制程度随药物浓度和作用时间的增加而增强。结论:A型K+通道参与Tca8113细胞增殖的过程,且A型K+通道阻滞剂能抑制Tca8113细胞的增殖
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