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- 2017
超短波对鼠骨髓间充质干细胞增殖及成骨能力的影响
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Abstract:
摘要 目的:探讨超短波对鼠骨髓间充质干细胞(rat bone marrow mesenchymal stem cells,rBMSCs)增殖及成骨分化能力的影响。方法:体外分离培养rBMSCs,取第2~4代rBMSCs置于超短波理疗仪上进行处理,1次/d,15 min/次,于1、4、7 d检测细胞增殖能力,3 d后检测细胞碱性磷酸酶(alkaline phosphatase,ALP)分泌量,7 d后检测成骨基因Runx2表达量。结果:每日给予超短波处理的实验组细胞经连续培养7 d后,与不经超短波处理的对照组相比,其增殖能力明显提升(P＜0.05),Runx2表达明显上调(P＜0.05),ALP分泌量增多; 结论:超短波能促进SD大鼠rBMSCs增殖和成骨分化
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