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- 2017
不同管径二氧化钛纳米管对骨髓间充质干细胞生物学行为的影响
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Abstract:
摘要 目的:研究不同管径二氧化钛纳米管(TNTs)对骨髓间充质干细胞(BMSCs)生物学行为的影响。方法:阳极氧化法分别制备管径30、100和200 nm的TNTs,MTT法检测细胞在材料表面粘附及增殖情况,碱性磷酸酶活性、胶原分泌量和细胞外基质矿化水平检测来评估细胞的成骨分化能力。结果:30 nm的TNTs促进BMSCs的早期粘附和增殖,但是其后期细胞增殖和成骨分化能力同对照组相比没有明显的统计学差异;200 nm的TNTs明显促进了BMSCs的成骨分化,但是其细胞粘附和增殖明显受到了抑制;100 nm的TNTs上BMSCs的早期粘附和增殖受到了轻微的抑制,但是随着时间的推移,其细胞增殖迎头赶上,而且其成骨分化能力显著大于对照组。结论:最适BMSCs生物学活性的应该是100 nm的TNTs
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