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- 2018
流体静压力对BMSCs/PRF复合构建的组织工程软骨移植物力学性能的影响
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Abstract:
摘要 目的: 观察流体静压力对骨髓间充质干细胞(bone marrow stem cells,BMSCs)复合富血小板纤维蛋白(platelet-rich fibrin,PRF)构建的组织工程软骨力学性能的影响。方法: 采用密度梯度离心法分离培养兔BMSCs并进行表面标志物鉴定,并制备细胞膜片。采用全血离心的方法分离兔PRF,构建BMSCs/PRF双膜复合体。将复合体随机分为3组:对照组、诱导组以及压力+诱导组。Real-time PCR检测成软骨标志基因Sox-9、Aggrecan 及 Col-II的表达量。HE染色、甲苯胺蓝染色观察复合体组织学改变。ElectroForce Systems3200力学测量仪测试BMSCs/PRF双膜复合体的弹性模量。结果: 诱导组及压力+成软骨分化诱导组中成软骨标志基因Sox-9、Aggrecan 及 Col-II的表达量显著高于对照组,且压力+诱导组中Aggrecan和Col-II的表达量显著高于压力组。HE染色发现,压力+诱导组中软骨细胞的数量较对照组及诱导组显著增高。甲苯胺蓝染色可见压力+诱导组中大量蓝紫色异染的细胞外基质。弹性模量检测发现,压力+诱导组的弹性模量显著高于对照组及诱导组。结论: 流体静压力可提高BMSCs/PRF双膜复合体构建组织工程软骨的质量及力学性能
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