|
|
- 2018
含锶/锌钙磷涂层对RAW264.7破骨细胞体外影响的研究
|
Abstract:
摘要 目的:研究锶或锌加入电化学沉积钙磷涂层后对类破骨细胞增殖和分化的影响。方法:以电化学沉积钙磷涂层为对照组,与加入锶或锌的电化学沉积钙磷涂层相比较。3种涂层分别接种RAW264.7细胞培养8 d,采用细胞计数试剂盒 (cell counting kit-8,CCK-8)检测细胞培养6 d后的增殖情况,同时检测细胞培养8 d后的抗酒石酸酸性磷酸酶 (tartrate resistant acid phosphatase,TRAP)活性。结果:3种涂层接种RAW264.7细胞后,使用CCK-8试剂盒检测,结果显示第4小时时加入锌的钙磷涂层其吸光度值明显高于其他两组,但第2天时的细胞增殖指数低于其他两组(P<0.05)。第14天的TRAP酶活性检测结果显示3组间比较差异无统计学意义。结论:含锌电化学沉积钙磷涂层促进破骨细胞的粘附,但加入锶或锌对类破骨细胞在电化学沉积钙磷涂层表面的增殖和分化无影响
| [1] | Mi B, Xiong W, Xu N, et al. Strontium-loaded titania nanotube arrays repress osteoclast differentiation through multiple signalling pathways: <i>in vitro</i> and <i>in vivo</i> studies [J]. Sci Rep, 2017, 7(1)∶2328 |
| [2] | 曹亚洲,程伟,胡勤刚.钛表面RGD/仿生磷酸钙复合涂层对MC3T3-E1细胞黏附、增殖、分化的影响[J].口腔医学研究,2017,33(7)∶693-697 |
| [3] | Wornham DP, Hajjawi MO, Orriss IR, et al. Strontium and osteoblast function [J]. Osteoporos Int, 2015, 26(8)∶2215 |
| [4] | Zhu S, Hu X, Tao Y, et al. Strontium inhibits titanium particle-induced osteoclast activation and chronic inflammation via suppression of NF-kappaB pathway [J]. Sci Rep, 2016, 6∶36251 |
| [5] | Boanini E, Torricelli P, Sima F, et al. Gradient coatings of strontium hydroxyapatite/zinc beta-tricalcium phosphate as a tool to modulate osteoblast/osteoclast response [J]. J Inorg Biochem, 2018, 183∶1-8 |
| [6] | Yang L, Perez-Amodio S, Barrere-De Groot FY, et al. The effects of inorganic additives to calcium phosphate on <i>in vitro</i> behavior of osteoblasts and osteoclasts [J]. Biomaterials, 2010, 31(11)∶2976-2989 |
| [7] | 秦博恒,杨犇,何东宁.载锌多壁碳纳米管/壳聚糖复合材料成骨性能的研究[J].口腔医学研究,2017,33(7)∶725-728 |
| [8] | Matsuike R, Tanaka H, Nakai K, et al. Continuous application of compressive force induces fusion of osteoclast-like RAW264.7 cells via upregulation of RANK and downregulation of LGR4 [J]. Life Sci, 2018, 201:30-36 |
| [9] | Wang P, Liu J, Yang Y, et al. Differential intensity-dependent effects of pulsed electromagnetic fields on RANKL-induced osteoclast formation, apoptosis, and bone resorbing ability in RAW264.7 cells [J]. Bioelectromagnetics, 2017, 38(8)∶602-612 |
| [10] | Azem FA, Delice TK, Ungan G, et al. Investigation of duty cycle effect on corrosion properties of electrodeposited calcium phosphate coatings [J]. Mater Sci Eng C Mater Biol Appl, 2016, 68∶681-686 |
