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- 2018
Sonic hedgehog参与人胰岛素样生长因子-1信号通路促使下颌骨髁突过度生长的实验研究
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Abstract:
摘要 目的:通过活体动物实验观察人胰岛素样生长因子-1(insulin-like growth factor,IGF-1) 与Sonic hedgehogxinh (Shh)介导的信号通路促进了髁突过度生长的作用。方法:制备IGF-1高表达表型转基因小鼠模型。将转基因鼠随机分为为5组:Cyclopamine刺激组;NVP-AEW541刺激组;IGF-1刺激组;Cyclopamine+IGF-1刺激组;生理盐水刺激组:空白对照,每组3只。随机选取一侧关节腔自31 d起隔天连续进行上述药物注射,共14 d,注射结束后收获实验侧髁突,固定,比较两侧髁突大小,分析差异性。结果:Cyclopamine刺激组及生理盐水刺激组实验侧与对照侧髁突大小比较差异无统计学意义。NVP-AEW541刺激组、IGF-1刺激组以及Cyclopamine+ IGF-1刺激组实验侧髁突小于对照侧髁突(P<0.05)。结论:IGF-1介导的信号通路促进了髁突肥大髁突的过度生长
| [1] | Xu L, Qi Y, Xu Y, et al. Co-inhibition of EGFR and IGF1R synergistically impacts therapeutically on adrenocortical carcinoma [J]. Oncotarget, 2016, 7(24)∶36235-36246 |
| [2] | Zhao J, Wang H, Hsiao CH, et al. Simultaneous inhibition of hedgehog signaling and tumor proliferation remodels stroma and enhances pancreatic cancer therapy [J]. Biomaterials, 2018, 159∶215-228 |
| [3] | 刘琦,魏小二,邹德荣,等.Mimics软件在髁突体积和表面积测量中的应用[J].口腔医学研究, 2017,33(4)∶404-408 |
| [4] | Fukaya S, Kanzaki H, Miyamoto Y, et al. Possible alternative treatment for mandibular asymmetry by local unilateral IGF-1 injection into the mandibular condylar cavity: experimental study in mice [J]. Am J Orthod Dentofacial Orthop, 2017, 152(6)∶820-829 |
| [5] | Nordstrand A, Bergstr？m SH, Thysell E, et al. Inhibition of the insulin-like growth factor-1 receptor potentiates acute effects of castration in a rat model for prostate cancer growth in bone [J]. Clin Exp Metastasis, 2017, 34(3-4)∶261-271 |
| [6] | Li R, Cai L, Ding J, et al. Inhibition of hedgehog signal pathway by cyclopamine attenuates inflammation and articular cartilage damage in rats with adjuvant-induced arthritis [J]. J Pharm Pharmacol, 2015, 67(7)∶963-971 |
| [7] | Olate S, Almeida A, Alister JP, et al. Facial asymmetry and condylar hyperplasia: considerations for diagnosis in 27 consecutives patients [J]. Int J Clin Exp Med, 2013, 6(10)∶937-941 |
| [8] | Meng Q, Long X, Deng M, et al. The expressions of IGF-1, BMP-2 and TGF-beta1 in cartilage of condylar hyperplasia [J]. J Oral Rehabi, 2011, 38(1)∶34-40 |
| [9] | Chen Y, Ke J, Long X, et al. Insulin-like growth factor-1 boosts the developing process of condylar hyperplasia by stimulating chondrocytes proliferation [J]. Osteoarthritis Cartilage,2012,20(4)∶279-287 |
| [10] | Suzuki S, Itoh K, Ohyama K. Local administration of IGF-1 stimulates the growth of mandibular condyle in mature rats [J]. J Orthod, 2004, 31(2)∶138-143 |
| [11] | Kojima I, Iikubo M, Kobayashi A, et al. High serum levels of IGF-I contribute to promotion of endochondral ossification in mandibular condyle and cause its specific elongation in acromegaly-like rats [J]. Horm Metab Res, 2008, 40(8)∶533-538 |
| [12] | Verhoeven TJ, Nolte JW, Maal TJ, et al. Unilateral condylar hyperplasia: a 3-dimensional quantification of asymmetry [J]. PLoS One, 2013, 8(3)∶e59391 |
| [13] | Vasconcelos BC, Goncalves F, Andrade A, et al. Mandibular asymmetry: literature review and case reportl [J]. Braz J Otorhinolaryngo, 2012, 78(4)∶137 |
| [14] | 张武阳,陈宇翔,杨璇璇,等.Shh信号通路对髁突肥大软骨细胞凋亡活性影响的研究[J].牙体牙髓牙周病学杂志,2017, 27(3)∶129-133 |
| [15] | Armstrong BE, Henner A, Stewart S, et al. Shh promotes direct interactions between epidermal cells and osteoblast progenitors to shape regenerated zebrafish bone [J]. Development, 2017, 144(7)∶1165-1176 |
