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-  2017 

FPS-ZM1拮抗晚期糖基化终末产物受体逆转高糖所致的牙周膜成纤维细胞炎症反应
FPS-ZM1 Rescues the Inflammatory Response of hPDLFs Caused by High Glucose via Inhibition of RAGE

DOI: 10.13701/j.cnki.kqyxyj.2017.11.006

Keywords: 牙周膜成纤维细胞,RAGE抑制剂,高糖,炎症反应,
hPDLFs
,RAGE inhibitor,High glucose,Inflammatory response

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Abstract:

摘要 目的:以体外培养的人牙周膜成纤维细胞(hPDLFs)为研究对象,探讨新型小分子RAGE特异性抑制剂对高糖环境培养下牙周膜成纤维细胞炎性因子的影响。方法:原代培养hPDLFs,Western-Blot检测高糖对RAGE表达的影响,RT-PCR检测FPS-ZM1对高糖环境下IL-6和TNF-α基因表达的影响,ELISA检测IL-6和TNF-α的蛋白分泌变化。结果:高糖可导致hPDLFs表面RAGE表达增加,加重细胞炎症反应。而FPS-ZM1可以下调IL-6和TNF-α的基因表达,同时减少炎症介质IL-6和TNF-α的分泌。结论:RAGE特异性抑制剂FPS-ZM1可以在一定程度上逆转高糖刺激导致hPDLFs产生的炎症反应

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