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- 2018
7,12-二甲基苯并[a]蒽诱发叙利亚地鼠颊鳞状细胞癌模型中Th1/Th2、Th17/Treg平衡变化研究
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Abstract:
摘要 目的: 采用叙利亚金黄地鼠在致癌剂7,12-二甲基苯并[a]蒽(7,12-dimethylbenz[a]anthracene,DMBA)的作用下,建立癌前病损以及口腔鳞状细胞癌(oral squamous cell carcinoma, OSCC)模型,检测空白对照组、阴性对照组、癌前病损组、OSCC组的地鼠脾脏中Th1、Th2、Th17、调节性T细胞 (regulatory T cell ,Treg)、Th1/Th2和Th17/Treg。分析上述细胞及比值比在组间的动态变化,探究平衡变化与口腔黏膜癌变的密切关系。方法: 1) 建模:利用1 mL 0.5%DMBA涂搽叙利亚金黄地鼠颊囊黏膜,1周3次,诱导癌前病损和OSCC模型。2) 取空白对照组(n=10)、阴性对照组(n=5)、癌前病损组(n=10)、OSCC组(n=18)脾脏,制备单细胞悬液后利用流式细胞仪检测。结果: 1)与空白对照组比较,口腔癌组Th1减少、Th2增加、Th17降低、Treg增加、Th1/Th2降低、Th17/Treg降低,差异均有统计学意义(P＜0.05)。2)与空白对照组比较,癌前病损组Th1减少、Th17减少、Treg增加、Th1/Th2降低、Th17/Treg降低,差异均有统计学意义(P＜0.05);而Th2增加,但差异无统计学意义(P＞0.05)。3)与癌前病损组比较,口腔癌组Th17/Treg升高,差异有统计学意义(P＜0.05);而Th1增加、Th2增加、Th17增加、Treg增加、Th1/Th2降低,差异均无统计学意义(P＞0.05)。结论: Th1/Th2平衡向Th2漂移可能参与了正常黏膜癌变的发生发展;Th17/Treg平衡向Treg漂移可能主要参与正常黏膜癌变的发生
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