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- 2018
氢氧化钙对人根尖牙乳头干细胞增殖的影响
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Abstract:
摘要 目的: 探讨氢氧化钙(calcium hydroxide,CH)对人根尖牙乳头干细胞(stem cells from apical papilla,SCAPs)增殖能力的影响。方法: 运用酶消化法结合组织块贴壁法分离培养SCAPs并加以鉴定。根据CH浸提原液的浓度不同分为6个实验组,在培养后的第1、3、5、7、9天分别终止培养,利用MTT法检测其吸光度(A)值,并进行统计学分析。结果: 与对照组比较,至第5天时,浓度为0.1%、0.5%、1.0%、5.0%组的SCAPs增殖比较差异无统计学意义(P>0.05),而浓度为10.0%和15.0%组已表现出对SCAPs增殖的抑制作用(P<0.05);到第7天和第9天时,所有实验组与对照组比较,均表现为对SCAPs的增殖抑制作用(P<0.05)。结论: CH对人SCAPs的增殖起抑制作用,并且随着时间延长其抑制作用增强,而高浓度CH对人SCAPs增殖的抑制作用更为明显
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