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- 2017
姜黄素和Dickkopf-1联用对口腔鳞状细胞癌细胞增殖和凋亡的影响研究
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Abstract:
摘要 目的:探讨姜黄素和分泌蛋白DKK1(Dickkopf-1)联用对口腔鳞状细胞癌细胞增殖和凋亡的影响。方法:CCK8实验检测0、5、10、20、40、80μmol/L的姜黄素作用于人口腔鳞状细胞癌CAL27、Tca8113、SCC-4细胞24、48、72 h的细胞增殖情况,计算IC50值;实验分为对照组、姜黄素组、Dickkopf-1组、姜黄素+Dickkopf-1组,各组细胞处理48 h后,CCK8试验检测细胞增殖,流式细胞仪检测细胞凋亡,Western blot检测Cleaved caspase3、β-catenin、CyclinD1蛋白表达。结果:不同浓度的姜黄素作用于CAL27、SCC-4、Tca8113细胞24、48、72 h后的细胞抑制率均显著高于0h时的细胞抑制率,且随着时间延长及浓度增加细胞抑制率增加(P<0.01),根据IC50值选择Tca8113细胞及30μmol/L的姜黄素作为后续研究。结论:姜黄素和Wnt/β-catenin信号通路抑制剂Dickkopf-1均能抑制口腔鳞状细胞癌细胞增殖和促进凋亡,两者联用的效果强于单独用姜黄素或Dickkopf-1
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