|
|
- 2017
血链球菌细菌素对牙龈卟啉单胞菌形貌及力学性质的影响
|
Abstract:
摘要 目的:研究血链球菌细菌素(简称血链素)对牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)形貌特征及力学性质的影响。方法:1)复苏、传代并鉴定血链球菌标准菌株ATCC10556、牙龈卟啉单胞菌标准菌株ATCC33277。2)将血链球菌低温高速离心、细胞破碎等方法提取血链素,使之作用于P.g,于37 ℃厌氧培养48 h。3)采用原子力显微镜(AFM)的接触模式,观察血链素作用前后P.g菌体的形貌变化。4)利用AFM测定血链素作用前后P.g的力-距离曲线,并计算黏附力及杨氏模量。结果:1)通过AFM的观察:P.g在血链素的作用下,菌体直径缩小、平均粗糙度及平均峰高度增加(P<0.05)。2)通过AFM的观察:血链素作用后,P.g的黏附力、杨氏模量显著降低(P<0.001)。正常P.g的黏附力平均值为(0.70±0.10) nN、杨氏模量平均值为(5.54±0.16) MPa;经血链素作用后P.g的黏附力平均值为(0.50±0.10) nN、杨氏模量平均值为(3.97±0.64) MPa。结论:1)血链素可引起牙龈卟啉单胞菌形貌特征发生改变。2)血链素可导致牙龈卟啉单胞菌的黏附力及杨氏模量降低
| [1] | Ma S,Li H,Yan C,et al. Antagonistic effect of protein extracts from Streptococcus sanguinis on pathogenic bacteria and fungi of the oral cavity [J]. Experimental and therapeutic medi- cine,2014,7(6):1486-1494 |
| [2] | 马晟利,董雪,夏雪.血链球菌细菌素对牙龈卟啉单胞菌生长曲线及细胞内钙离子浓度的影响[J].口腔医学研究,2014,30(2):108-111 |
| [3] | Umemoto T,Hamada N. Characterization of biologically activecell surface components of a periodontal pathogen:the roles of major and mi minor fimbriae of Porphyromonas gingivalis [J]. JPeriodontol,2003,74(1):119-122 |
| [4] | Gaboriaud E, Dufrene YF. Atomic force mircr-oscopy of microbial cells: Application to nano- mechanical properties,surface forces and mol- ecular recognition forces [J]. Colloid surface surfBioint Biointerfaces,2007,54(1):10-19 |
| [5] | 史彦,李成杰,张明正.龋齿及牙周病患者与健康人群口腔微生态的比较研究[J].口腔医学研究,2016,32(12):1265-1268 |
| [6] | 马晟利,王丹,闰闯.血链球菌胞内蛋白与胞外蛋白分别对中间普氏菌与牙龈卟啉单胞菌生物膜作用的实验研究[J].口腔医学研究,2012,28(3):206-209 |
| [7] | Li QS, Lee GY, Ong CN, et al. AFM indentatio tion study of breast cancer cell [J]. Biochem Bi- ophys Res Commun, 2008, 374(4):609-613 |
| [8] | Wirtz D, Konstantopoulos K, Searson PC. The physics of cancer: the role of physical interact- ions and mechanical forces in metastasis [J]. Nature Reviews Cancer, 2011, 11(7): 512-522 |
| [9] | Garcia R, Knoll A W, Riedo E. Nature Nanote-chnology, 2014, 9:577 |
| [10] | 宋红,马秦,陈永进,等.牙龈卟啉单胞菌红细胞凝集素A黏附和入侵人牙龈上皮细胞的研究[J].实用口腔医学杂志2007,23(3):700-703 |
| [11] | Lopez-polin G, Gomez-Navarro C, ParenteV, et al. Nature Physics,2015,11:26 |
