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- 2018
间充质干细胞表面标记在颞下颌关节疾病患者滑膜间充质细胞表达的比较
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Abstract:
摘要 目的:检测间充质干细胞表面标记(CD73、 CD90、CD105、CD271和CD34)在人颞下颌关节滑膜间充质细胞(synovial mesenchymal cells,SMCs)上的表达;同时比较这些标记在髁突肥大滑膜无病变和颞下颌关节骨关节病伴关节盘穿孔患者SMCs上表达水平的差异。方法:分别采用多次贴壁培养和组织块法分离得到髂骨骨髓间充质干细胞(mesenchymal stem cells,MSCs)和颞下颌关节原代SMCs并传至3代,应用流式细胞仪检测CD34、CD73、 CD90、CD105、CD271的表达水平。结果:髂骨MSCs及颞下颌关节SMCs表面CD73、CD90、CD105表达量均>90%,CD271表达量<1% ,CD34表达量较低为0.47%~30.00%。结论:髂骨MSCs和颞下颌关节SMCs高表达CD73、CD90、CD105,CD271和CD34表达量较低;CD34在人颞下颌关节骨关节病患者SMCs上的表达量较髁突肥大滑膜无病变患者高
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