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- 2018
羧甲基壳聚糖-溶菌酶纳米凝胶稳定无定形磷酸钙促进脱矿牙釉质再矿化
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Abstract:
摘要 目的:制备羧甲基壳聚糖(CMC)-溶菌酶(LYZ)凝胶,稳定无定形磷酸钙(ACP)进而促进脱矿牙釉质再矿化。方法:首先制备CMC/LYZ-ACP纳米凝胶,利用透射电镜(TEM)观察凝胶的形貌,X射线能谱元素成像分析技术(EDX-Mapping)进行关键元素的定量及分布的分析。然后将纳米凝胶涂抹于酸蚀的牙釉质表面,利用扫描电镜(SEM)、纳米压痕实验(nano-idention)对脱矿牙釉质再矿化效果进行评估,并与人工唾液组进行对照。结果:CMC/LYZ-ACP纳米凝胶为粒径约50~300 nm的致密的球形颗粒。扫描电镜下可见脱矿后的釉质表面有新的晶体形成,与对照组相比,实验组的硬度及弹性模量更接近正常牙釉质。结论:CMC/LYZ-ACP纳米凝胶可以用于脱矿牙釉质的再矿化,属于新型的仿生再矿化材料
| [1] | Dorri M, Dunne SM, Walsh T, et al. Micro-invasive interventions for managing proximal dental decay in primary and permanent teeth [J]. Cochrane Database Syst Rev, 2015, 11 (11)∶CD010431 |
| [2] | Moradian-Oldak J. Protein-mediated enamel mineralization [J]. Front Biosci (Landmark Ed), 2012, 17(6)∶1996-2023 |
| [3] | 樊明文.龋病微创治疗[J]. 中国实用口腔科杂志, 2012 , 5 (8)∶453-455 |
| [4] | 杨国斌,李袁聂.MMP-9基因敲除小鼠磨牙牙胚中釉原蛋白和成釉蛋白表达的研究[J].口腔医学研究,2016,32(11)∶1117-1121 |
| [5] | Li Z, Xu W, Zhang C, et al. Self-assembled lysozyme/carboxymethylcellulose nanogels for delivery of methotrexate [J]. Int J Biol Macromol, 2015, 75∶166-172 |
| [6] | Zhang LX, Cao XH, Zheng YB, et al. Covalent modification of single glass conical nanopore channel with 6-carboxymethyl-chitosan for pH modulated ion current rectification [J]. Electrochemistry Communications, 2010, 12(9)∶1249-1252 |
| [7] | Ma Y, Zhang J, Guo S, et al. Biomimetic mineralization of nano-sized, needle-like hydroxyapatite with ultrahigh capacity for lysozyme adsorption [J]. Mater Sci Eng C Mater Biol Appl, 2016, 68∶551-556 |
| [8] | Dahoumane SA, Jeffryes C, Mechouet M, et al. Biosynthesis of inorganic nanoparticles: a fresh look at the control of shape, size and composition [J]. Bioengineering (Basel), 2017, 4(1)∶14 |
| [9] | Tay FR, Pashley DH. Guided tissue remineralisation of partially demineralised human dentine [J]. Biomaterials, 2008, 29(8)∶1127-1137 |
| [10] | Chen Z, Cao S, Wang H, et al. Biomimetic remineralization of demineralized dentine using scaffold of CMC/ACP nanocomplexes in an <i>in vitro</i> tooth model of deep caries [J]. PLoS One, 2015, 10(1)∶e0116553 |
