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- 2018
口腔鳞状细胞癌外泌体mRNA表达图谱分析
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Abstract:
摘要 目的: 研究口腔鳞状细胞癌细胞(oral squamous cell cancer cells, OSCC))外泌体与正常口腔上皮细胞(Human immortalized oral epithelial cell, HOEC)外泌体mRNA之间的表达差异,通过特异性外泌体mRNA表达图谱分析,为探究口腔鳞状细胞癌的发生发展机制打下一定的研究基础。方法: 利用高通量测序技术检测CAL-27和HOEC外泌体mRNA表达谱,筛选出差异mRNA,对差异mRNA进行GO(Gene Ontoloty)分析和KEGG通路分析。结果: 口腔鳞状细胞癌细胞外泌体中检测到mRNA 44699 条,口腔上皮细胞外泌体中mRNA数量为44761条,其中,差异表达的mRNA共471条(234个上调,237个下调)。生物信息学分析表明上述差异表达的mRNA参与了口腔癌的生物学调节功能和通路。结论: 口腔鳞癌细胞及口腔上皮细胞均能产生丰富的外泌体;口腔鳞状细胞癌细胞外泌体与正常口腔上皮细胞外泌体间mRNA表达谱存在较大差异,差异表达的mRNA在肿瘤的发生、发展及转移过程中可能有重要作用
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