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- 2015
银杏叶提取物对骨髓间充质干细胞增殖及成骨分化的影响
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Abstract:
摘要 目的:研究银杏叶提取物(ginkgo biloba extract ,GBE)对大鼠的骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)增殖及成骨分化过程的影响。方法:将不同浓度的GBE作用于第3代BMSCs,通过CCK-8(Cell Counting Kit-8)法及流式细胞仪法检测其对细胞增殖活性变化的影响,通过茜素红染色及碱性磷酸酶(Alkalinet phosphatase,ALP)法判定其对细胞成骨能力的影响。RT-PCR检测成骨相关基因骨形成蛋白2(bone morphogenetic protein-2, BMP-2)、Runx2(runt-related transcription factor2)的mRNA的表达。结果:不同浓度的GBE对BMSCs的增殖无明显影响,但能使细胞处于S期,显著促进ALP的表达,在浓度为150 mg/L时表达最多。其成骨相关基因BMP-2、Runx2的mRNA表达也相对增高。结论:银杏叶提取物对骨髓间充质干细胞的成骨分化具有积极意义
| [1] | Yeong Shik Kim, Mi Kyung Pyo, Kyung Mi Park, et al. Antiplatelet and Antithrombotic Effects of a Combination of Ticlopidine and Ginkgo bilobaExt (EGb 761). Thrombosis Research, 1998, 33-38 |
| [2] | Christophe Prouillet, Jean-Claude Mazie `re,Ce cile Mazie`re,et al. Stimulatory effect of naturally occurring flavonols quercetin and kaempferol on alkaline phosphatase activity in MG-63 human osteoblasts through ERK and estrogen receptor pathway. Biochemical Pharmacology, 2004, 67(7)∶1307-1313 |
| [3] | Oh SM, Chung KH. Estrogenic activities of Ginkgo biloba extracts. Life Sci, 2004, 74(11)∶1325-1335 |
| [4] | 李松,郭延伟,房殿吉.兔下颌骨来源骨髓间充质干细胞生物学行为及其体内成骨的研究[J].口腔医学研究,2013,(2)∶123-127 |
| [5] | Kode JA, Mukherjee S, Joglekar MV, et al. Mesenchymal stem cells: immunobiology and role in immunomodulation and tissue regeneration. Cytotherapy, 2009, 11(4)∶377-391 |
| [6] | Morikawa S, Mabuchi Y, Kubota Y, et al. Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow. J Exp Med, 2009, 206(11)∶2483-2496 |
| [7] | Sara Castiglioni, Alessandra Cazzaniga, Walter Albisetti, et al. Magnesium and Osteoporosis: Current State of Knowledge and Future Research Directions. Nutrients, 2013, 5(8)∶3022-3033 |
| [8] | Rossouw JE,Anderson GL,Prentice RL, et al. Risks and benefitis of estrogen plus progestin in healthy postmenopausal women:principal results From the Women’s Health Initiative randomized controlled trial[J],JAMA,2002,288(3)∶321-333 |
| [9] | Komori T. Regulation of bone development and maintenance by Runx2.Front BioscI, 2008, 13(3)∶898-903 |
| [10] | de Mattos Carvalho A, Alves AL, Golim MA,et al. Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue. Vet Immunol Immunopathol, 2009, 132(2-4)∶303-306 |
| [11] | Seung Min Oh, Ha Ryong Kim, and Kyu Hyuck Chung. Effects of Ginkgo Biloba on In Vitro Osteoblast Cells and Ovariectomized Rat Osteoclast Cells. Arch Pharm Res Vol 31, 2008, 31(2)∶ 216-224 |
| [12] | Phimphilai M, Zhao Z, Boules H, et al. BMP signaling is required for RUNX2-dependent induction of the osteoblast phenotype. J Bone Miner Res,2006,21(4)∶637-646 |
