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- 2017
周期性流体静压力对BMSCs细胞增殖活性及超微结构的影响
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Abstract:
摘要 目的:观察周期性流体静压力作用下骨髓间充质干细胞(Bone Marrow Stem cells,BMSCs)细胞增殖活性及超微结构的改变。方法:贴壁法分离培养大鼠BMSCs。采用自主研发的流体静压力加载装置对大鼠BMSCs进行力学加载,加载压力为0~45 kPa、0~90 kPa,加载频率为0.1 Hz,时间1 h,连续加载2 d。对照组细胞不加压。采用CCK8法检测细胞的增殖活性,采用透射电镜观察大鼠BMSCs超微结构的变化。结果:0~45 kPa 、0~90 kPa周期性流体静压力作用下BMSCs增殖活性显著高于对照组(P<0.05),透射电镜下观察发现,0~45 kPa作用下BMSCs细胞内质网轻度扩张,胶原合成量增加。而0~90 kPa作用下细胞出现了大量胶原及凋亡小体。结论:适宜的周期性流体静压力作用下可提高细胞的增殖活性,但过高的压力刺激可诱导细胞产生凋亡
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