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-  2016 

白消安对雄性青春期小鼠的生殖系统发育的影响 Effects of Busulfan on Reproductive System Development of Male Puberty Mouse

Keywords: 白消安,BALB/c小鼠,生殖,形态学,性行为

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Abstract:

目的:研究白消安对雄性青春期小鼠生殖系统发育的影响。方法:108只雄性BALB/c小鼠随机分为对照组(n=24)和4个给药剂量的实验组(n=84)(10mg/kg 1次组,A组;10mg/kg 2次组,B组;20mg/kg组,C组;30mg/kg组,D组)。测量睾丸重量,HE染色观察睾丸组织形态学,并观察小鼠性行为和生育能力。结果:(1)组织形态学:第4周,各实验组的睾丸重量,B组的完全生精比例,B组、C组和D组的无生精比例与对照组相比有显著差异(P<0.05);第8周,C组的完全生精比例,D组的睾丸重量、完全生精比例和无生精比例与对照组相比有显著差异(P<0.05)。第12周,C组的睾丸重量,D组的睾丸重量、完全生精情况和无生精情况与对照组相比有显著差异(P<0.05)。(2)性行为学观察:C组和D组合并后与对照组相比,爬跨率和生育率存在显著性差异(P<0.05)。结论:白消安可损害睾丸组织发育,其毒性效应具有量效性和时效性

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