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-  2017 

抑制MRN复合体功能对喉鳞状细胞癌放射敏感性的影响 Influences of Functional Inhibition of MRN Complex on Radiosensitivity of Laryngeal Squamous Cell

Keywords: MRN复合体,放射敏感性,Nbs1基因,RNA干扰,喉鳞癌

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Abstract:

目的:探讨抑制Nbs1基因的表达对喉鳞癌细胞放射敏感性的影响。方法:用ADV1包装的干扰质粒(ADV1-Nbs1)和空载腺病毒(ADV1-NC)转染Hep-2细胞,将细胞分为正常对照组、ADV1-Nbs1转染组、ADV1-NC转染组,上述各组又分为X射线照射组和未照射组。RT-PCR和Western Blot检测Nbs1基因的表达水平,MTT法描绘出各组增殖曲线,流式细胞仪检测细胞周期,RT-PCR法测定各组相对端粒长度。结果:RT-PCR和Western Blot结果显示ADV1-Nbs1转染组的Nbs1基因的表达水平明显低于正常对照组和ADV1-NC转染组。ADV1-Nbs1转染联合X射线放射组抑制增殖最显著(P<0.05)。对比正常对照组(11.8%),ADV1-Nbs1转染组和ADV1-Nbs1转染联合放射组G_2/M期百分比均有增高,分别为34.2%(P<0.05)、25%(P<0.05)。ADV1-Nbs1转染组端粒长度较正常组缩短约57%(P<0.05),ADV1-Nbs1转染组同ADV1-Nbs1转染联合放射组端粒长度无差异(P>0.05)。结论:干扰MRN复合体功能可有效使Hep-2细胞的放射敏感性提高

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