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-  2017 

胡黄连苷Ⅱ对大鼠肾小管上皮细胞缺氧/复氧损伤的保护作用 Effect of Picroside Ⅱ on Renal Tubular Epithelial Cells with Hypoxia Reoxygenation

Keywords: 胡黄连苷Ⅱ,肾小管上皮细胞,缺氧/复氧,氧化应激

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Abstract:

目的:研究胡黄连提取物对缺氧/复氧后肾小管上皮细胞氧化应激的保护作用和机制。方法:采用小鼠肾小管上皮细胞建立缺氧/复氧损伤模型,分别用不同梯度浓度胡黄连苷Ⅱ(1,10,100,1 000μg/ml)进行药物干预。MTT法检测细胞活性,BCA蛋白质分析试剂盒检测细胞培养液中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,免疫组化检测Bax蛋白的表达,Western Blot法检测高迁移率族蛋白(HMGB1)的表达,Realtime PCR检测Bax和Bcl-2mRNA表达。结果:与正常组相比,各缺氧/复氧组细胞内氧化应激水平增高,凋亡细胞或死亡细胞数量明显增加。与单纯缺氧/复氧组比较,不同浓度梯度的胡黄连苷Ⅱ可抑制细胞内的氧化损伤。胡黄连苷Ⅱ使肾小管上皮细胞增殖活性增强,细胞凋亡数量减少,培养液中MDA含量下降、SOD活性增强,Bax蛋白和HMGB1蛋白表达下调,Bax mRNA水平下调,Bcl-2mRNA水平上调。结论:缺氧/复氧导致肾小管上皮细胞内氧化应激增强,胡黄连苷Ⅱ能通过抑制肾小管细胞内氧化应激来改善缺氧/复氧后的细胞凋亡和坏死

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