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-  2017 

Nrf2调控的炎症介质表达在肠缺血再灌注所致肾脏损伤中的作用 Role of Inflammatory Mediators Regulated by Nrf2 in Intestinal Ischemia-Reperfusion Induced Kidney Injury in Rats

Keywords: 急性肾损伤,肠缺血再灌注,肠系膜上动脉,炎症因子

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Abstract:

目的:探讨Nrf2调控的炎症介质表达在肠缺血/再灌注所致肾脏损伤中的作用。方法:健康雄性SPF级C57BL/6J小鼠36只,随机均分为如下3组:假手术组(S组)、缺血再灌注组(I/R组)、拮抗剂Brusatol+缺血再灌注组(Brusatol+I/R组)。复制小鼠肠缺血再灌注模型,于再灌注2h时采集颈动脉血样,然后处死小鼠,取肾组织,测定血清尿素氮(BUN)、肌酐(Cr)和中性粒细胞明胶酶相关脂质运载蛋白(NGAL)水平,检测肾组织Nrf2蛋白表达,血清肿瘤坏死因子α(TNF-α)、白细胞介素-6、10(IL-6、IL-10)的含量。显微镜下观察肾组织病理学结果,并行病理学损伤评分。结果:与S组比较,I/R组肾脏组织病理学损伤评分升高(P<0.05),血清BUN、Cr和NAGL浓度升高,肾脏组织Nrf2蛋白表达上调,血清促炎因子IL-6、TNF-α在I/R组显著增高(P<0.05),抗炎因子IL-10的含量在I/R组表达显著降低(P<0.05)。使用Brusatol后,I/R组肾脏组织病理学损伤评分进一步增高(P<0.05),血清BUN、Cr和NAGL浓度进一步增高(P<0.05),肾脏组织Nrf2蛋白表达下降(P<0.05),Brusatol+I/R组血清促炎因子IL-6、TNF-α较I/R组进一步增高(P<0.05),抗炎因子IL-10的含量在I/R组表达更加降低(P<0.05)。结论:Nrf2调控的炎症介质表达在肠缺血再灌注所致肾脏损伤病程进展中的作用机制可能为调节抗炎因子/促炎因子平衡,进而调控远隔肾脏损伤

References

[1]  Braun S,Hanselmann C,Gassmann MG,et al.Nrf2transcription factor,a novel target of keratinocyte growth factor action which regulates gene expression and inflammation in the healing skin wound[J].Mol Cell Biol,2002,22(15):5 492-5 455.
[2]  Teke Z,Sacar M,Yenisey C,et al.Activated protein C attenuates intestinal reperfusion-induced acute lung injury:an experimental study in a rat model[J].Am J Surg,2008,195(6):861-873.
[3]  Ren D,Villeneuve NF,Jiang T,et al.Brusatol enhances the efficacy of chemotherapy by inhibiting the Nrf2-mediated defense mechanism[J].Proc Natl Acad Sci USA,2011,108:1 433-1 438.
[4]  Arisawa T,Tahara T,Shibata T,et al.The relationship between Helicobacter pylori infection and promoter polymorphism of the Nrf2gene in chronic gastritis[J].Int J Mol Med,2007,19(1):143-148.
[5]  Ma Q,Battelli L,Hubbs AF.Multiorgan autoimmune inflammation,enhanced lymphoproliferation,and impaired homeostasis of reactive oxygen species in mice lacking the antioxidant-activated transcription factor Nrf2[J].Am J Pathol,2006,168(6):1 960-1 974.
[6]  Sun Q,Meng QT,Jiang Y,et al.Ginsenoside Rb1attenuates intestinal ischemia reperfusion induced renal injury by activating Nrf2/ARE pathway[J].Molecules,2012,17:7 195-7 205.
[7]  Masson-Lecomte A,Rava M,Real FX,et al.Inflammatory Biomarkers and Bladder Cancer Prognosis:A Systematic Review[J].European Urology,2014,66(6):1 078-1 091.
[8]  Gammon CS,Kruger R,Conlon CA,et al.Inflammatory status modulates plasma lipid and inflammatory marker responses to kiwifruit consumption in hypercholesterolaemic men[J].Nutr Metab Cardiovasc Dis,2014,24(1):91-99.
[9]  Mohsenzadeh Y,Rahmani A,Cheraghi J,et al.Prenatal exposure to nicotine in pregnant rat increased inflammatory marker in newborn rat[J].Mediators of Inflamm,2014,2014(5):209-218.
[10]  Mao YF,Zheng XF,Cai JM,et al.Hydrogen-rich saline reduces lung injury induced by intestinal ischemia/reperfusion in rats[J].Biochem Biophys Res Commun,2009,381:602-605.
[11]  Gong P,Stewart D,Hu B,et al.Activation of the mouse heme oxygenase-1gene by 15-deoxy-Delta(12,14)-prostaglandin J(2)is mediated by the stress response elements and transcription factor Nrf2[J].Antioxid Redox Signal,2002,4:249-257.
[12]  Mcwhinnie DL,Thompson JF,Taylor HM,et al.Morphometric analysis of cellular infiltration assessed by monoclonal antibody labeling in sequential human renal allograft biopsies[J].Transplantation,1986,42(4):352-358.

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