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-  2018 

BRD4抑制剂对前列腺癌细胞增殖与凋亡的作用及其机制 BRD4 Inhibitor Suppresses Cell Proliferation and Induces Apoptosis in Prostate Cancer

Keywords: JQ1,前列腺癌,C-MYC

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Abstract:

目的:探讨BRD4抑制剂JQ1对前列腺癌细胞增殖的影响及其作用机制。方法:JQ1浓度分别为0.016,0.08,0.4,2,10,20,50μmol/L处理前列腺癌细胞DU145,在24,48,72h用CCK8法检测JQ1对前列腺癌细胞DU145增殖的影响并计算JQ1在前列腺癌细胞DU145的IC50值,之后选取JQ1浓度为10,20μmol/L。流式细胞仪检测JQ1对前列腺癌细胞株DU145凋亡及细胞周期的影响,细胞划痕实验检测JQ1对前列腺癌细胞DU145迁移能力的影响,Western Blot法检测癌基因C-MYC蛋白表达量。结果:浓度为10,20μmol/L的JQ1抑制前列腺癌细胞DU145的增殖及迁移,促进前列腺癌细胞凋亡,阻滞细胞于G0/G1期,下调C-MYC蛋白表达量。结论:JQ1可以抑制前列腺癌细胞株的生长,其作用机制可能与JQ1下调癌基因C-MYC表达有关

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