全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
-  2018 

商陆皂苷甲在大鼠肾缺血再灌注损伤中的作用 Effect of Esculentoside A on Renal Ischemia-Reperfusion Injury in Rats

Keywords: 商陆皂苷甲,肾缺血再灌注损伤,白细胞介素17A,白细胞介素6,钠-钾ATP酶

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的:探讨商陆皂苷甲(EsA)对大鼠肾缺血再灌注(I/R)损伤的影响及机理。方法:SD大鼠60只,随机分为对照组(C组)、缺血再灌注组(I/R组)和缺血前EsA预处理组(EsA组),每组20只;采用夹闭双侧肾蒂30min后再灌注的方法制备肾缺血再灌注损伤大鼠模型,检测大鼠血清和肾组织样本中血肌酐(SCr)、血尿素氮(BUN)、IL-17A、IL-6及钠-钾ATP酶水平,HE染色后评估肾小管损伤程度。结果:与对照组比较,EsA组大鼠术前摄水量增多、摄食减少(P<0.05);再灌注12h后,与对照组比较,I/R组大鼠SCr、BUN水平升高(P<0.05),EsA组无显著性差异(P>0.05);与对照组比较,I/R组大鼠肾组织IL-17A、IL-6表达水平升高(P<0.05),钠-钾ATP酶表达水平下降(P<0.05),EsA组无显著性差异(P>0.05);C组、I/R组和EsA组肾小管损伤评分为3.02±1.13、10.53±2.13和9.85±1.91,EsA组较I/R组评分降低(P<0.05)。结论:EsA预处理能降低大鼠肾缺血再灌注时的损伤程度,发挥保护作用,其机制与减少炎症因子释放、增加钠-钾ATP酶表达有关

References

[1]  Ma J,Chen Q,Lai D,et al.Separation and purification of triterpene saponins from roots of Radix phytolaccae by high-speed countercurrent chromatography coupled with evaporative light scattering detection[J].J Liq Chromatogr Relat Technol,2010,33(4):563-571.
[2]  彭波,李华,王育斌.结直肠癌患者癌组织中IL-17A和IL-17F的表达及意义[J].武汉大学学报:医学版,2013,34(3):388-390;406.Peng B,Li H,Wang YB.Expression and significance of IL-17Aand IL-17Fin human colorectal cancer[J].Medical Journal of Wuhan University,2013,34(3):388-390;406.
[3]  王铁军,郭洪宝,卢迪,等.商陆中商陆皂苷甲的含量测定[J].中国中药杂志,2008,33(2):143-145.Wang TJ,Guo HB,Lu D,et al.Determination of esculentoside A content in the Phytolaccae[J].China J Chin Mater Med,2008,33(2):143-145.
[4]  崔楠楠,孟祥龙,马俊楠,等.商陆皂苷甲急性毒性与利尿作用研究[J].医药导报,2014,33(8):981-984.Cui NN,Meng XL,Ma JN,et al.Study on acute toxic and diuretic effects of esculentoside A[J].Herald of Medicine,2014,33(8):981-984.
[5]  易文龙,郭晓轶,樊舒豪,等.商陆皂甙甲对大鼠的体外杀精作用[J].河南新乡医学院学报,2014,31(9):688-690.Yi WL,Guo XQ,Fan SH,et al.The spermicidal effect of Esculentoside A on rats in vitro[J].J Xinxiang Med Coll,2014,31(9):688-690.
[6]  Gonul Y,Kazandi S,Kocak A,et al.Interleukin-18binding protein pretreatment attenuates kidney injury induced by hepatic ischemia reperfusion[J].Am J Med Sci,2016,352(2):200-207.
[7]  Barut F,Ozacmak VH,Turan I,et al.Reduction of acute lung injury by administration of spironolactone after intestinal ischemia and reperfusion in rats[J].Clin Invest Med,2016,39(1):E15-E24.
[8]  国家药典委员会.中华人民共和国药典(2015版)[M].北京:中国医药科技出版社,2015:324-325.Chinese Pharmacopoeia Commission.Pharmacopoeia of China(2015)[M].Peking:China Medical Science Press,2015:324-325.
[9]  Hu Z,Qiu L,Xiao Z,et al.Effects of esculentoside A on autoimmune syndrome induced by Campylobacterjejuni in mice and its modulation on T-lymphocyte proliferation and apoptosis[J].Int Immunopharmacol,2010,10(1):65-71.
[10]  贾金萍,邢婕,秦雪梅.中药商陆利尿作用的实验研究[J].山西医科大学学报,2014,45(8):725-728.Jia JP,Xing J,Qin XM.Experiment research of diuretic effect of Phytolacca Americana L[J].J Shanxi Med Univ,2014,45(8):725-728.
[11]  Wang Z,Ren L,Lei L,et al.The relationship between neutrophil counts on admission and angiographic no-reflow after primary percutaneous coronary intervention in patients with ST-segment elevation myocardial infarction[J].Acta Cardiol,2016,71(2):241-246.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133