全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
-  2017 

细胞焦亡在糖尿病心肌缺血再灌注损伤的变化及作用机制 Changes and Mechanism of Pyroptosis in Myocardial Ischemia-Reperfusion in Diabetic Rats

Keywords: 糖尿病,心肌缺血再灌注损伤,半胱天冬酶-1,细胞焦亡

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的:探究细胞焦亡在糖尿病大鼠心肌缺血再灌注损伤的变化及作用机制。方法:选择成年健康雄性SD大鼠,体重210-230g。大鼠糖尿病模型采用腹腔注射1%链脲佐菌素60mg/kg制备,取造模成功的SD大鼠40只,将其随机分为2组(n=20):糖尿病假手术组(DS组)、糖尿病心肌缺血再灌注组(DIR组);另取非糖尿病大鼠40只,将其随机分为2组(n=20):假手术组(NS组)、心肌缺血再灌注组(NIR组)。采用结扎左冠状动脉前降支30min再灌注120min的方法制备心肌缺血再灌注模型;NS组、DS组只穿线不结扎。再灌注结束后,采集动脉血样,检测肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)的活性;采用TTC法检测心肌梗死面积;HE染色观察心肌组织病理学变化;Western Blot法检测NLRP3、caspase-1、凋亡相关斑点样蛋白(ASC)和IL-1β在心肌组织的表达。结果:DS与NS相比,NLRP3、caspase-1、ASC和IL-1β蛋白表达增加;非糖尿病和糖尿病大鼠在心肌缺血再灌注时,CK-MB和LDH的活性增加,NLRP3、caspase-1、ASC和IL-1β蛋白表达增加;DIR组与NIR相比,心脏病理学损伤加重,心肌梗死面积增加,CK-MB和LDH活性增高,NLRP3、caspase-1、ASC和IL-1β蛋白表达增加。结论:糖尿病大鼠心肌对缺血再灌注敏感性增加,缺血再灌注损伤加重,其机制可能与NLRP3介导的caspase-1依赖性细胞焦亡的作用密切相关

References

[1]  Luo B,Li B,Wang W,et al.NLRP3Gene Silencing Ameliorates DiabeticCardiomyopathy in a Type 2Diabetes Rat Model[J].PLoS One,2014,9(8):e104771.
[2]  Byrne BG,Dubuisson JF,Joshi AD,et al.Inflammasome components coordinate autophagy and pyroptosis as macrophage responses to infection[J].M Bio,2013,4(1):e00620.
[3]  Li X,Du N,Zhang Q,et al.MicroRNA-30dregulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy[J].Cell Death Dis,2014,5:e1479.
[4]  Yang JR,Yao FH,Zhang JG,et al.Ischemia-reperfusion induces renal tubule pyroptosis via the CHOPcaspase-11pathway[J].Am J Physiol Renal Physiol,2014,306(1):F75-F84.
[5]  Li H,Liu Z,Wang J,et al.Susceptibility to myocardial ischemia reperfusion injury at early stage of type 1diabetes in rats[J].Cardiovasc Diabetol,2013,12:133.
[6]  Sandanger,Ranheim T,Vinge LE,et al.The NLRP3inflammasome is up-regulated in cardiac fibroblasts and mediates myocardial ischaemia-reperfusion injury[J].Cardiovasc Res,2013,99(1):164-174.
[7]  Takahashi M.NLRP3inflammasome as a novel player in myocardial infarction[J].Int Heart J,2014,55(2):101-105.
[8]  Keller PF,Carballo D,Roffi M.Diabetes and acute coronary syndrome[J].Minerva Med,2010,101(2):81-104.
[9]  Liu Y,Yang H,Liu LX,et al.NOD2contributes to myocardial ischemia/reperfusion injury by regulating cardiomyocyte apoptosis and inflammation[J].Life Sci,2016,149:10-17.
[10]  Doitsh G,Galloway NL,Geng X,et al.Cell death by pyroptosis drives CD4T-cell depletion in HIV-1infection[J].Nature,2014,505(7 484):509-514.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133